Recombinant
RabMAb

Recombinant Anti-Slit2 antibody [SP122] - BSA and Azide free (ab242404)

Overview

  • Product name

    Anti-Slit2 antibody [SP122] - BSA and Azide free
    See all Slit2 primary antibodies
  • Description

    Rabbit monoclonal [SP122] to Slit2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide within Human Slit2 aa 1450 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: O94813

  • Positive control

    • IHC-P: Human prostate adenocarcinoma tissue. Flow Cyt: SH-SY5Y cells.
  • General notes

    Ab242404 is the carrier-free version of ab184856. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab242404 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab242404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.
  • Tissue specificity

    Fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined.
  • Sequence similarities

    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 7 EGF-like domains.
    Contains 1 laminin G-like domain.
    Contains 20 LRR (leucine-rich) repeats.
    Contains 4 LRRCT domains.
    Contains 4 LRRNT domains.
  • Domain

    The leucine-rich repeat domain is sufficient for guiding both axon projection and neuronal migration, in vitro.
  • Cellular localization

    Secreted. The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated.
  • Information by UniProt
  • Database links

  • Alternative names

    • Drad 1 antibody
    • E030015M03Rik antibody
    • E130320P19Rik antibody
    • FLJ14420 antibody
    • OTTHUMP00000158695 antibody
    • OTTHUMP00000217852 antibody
    • OTTHUMP00000217853 antibody
    • OTTHUMP00000217854 antibody
    • Slil 3 antibody
    • Slil3 antibody
    • Slit 2 antibody
    • Slit homolog 2 (Drosophila) antibody
    • Slit homolog 2 antibody
    • Slit homolog 2 protein antibody
    • Slit homolog 2 protein C-product antibody
    • Slit-2 antibody
    • Slit2 antibody
    • SLIT2_HUMAN antibody
    see all

Images

  • Flow Cytometry analysis of Neuro-2a (mouse neuroblastoma) cells labeling SLIT2 with purified ab184856 at 1:80 dilution (10.3 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab125938) / Black. Unlabeled control - Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide�(ab184856)
  • Flow cytometric analysis of rabbit anti-Slit2 (SP122) antibody ab184856 diluted 1/100 in SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells (green) compare to negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab184856)

  • Staining of Slit2 in a Formalin/PFA-fixed paraffin-embedded section of human prostate adenocarcinoma tissue using ab184856 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab184856)

References

ab242404 has not yet been referenced specifically in any publications.

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