Overview

  • Product name

  • Description

    Rabbit polyclonal to SLK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human SLK (internal sequence). The exact sequence is proprietary.
    Database link: Q9H2G2

  • Positive control

    • WB: A549 and H1299 whole cell extracts. ICC/IF: H1299 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab226986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 143 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    SLK is a serine/threonine protein kinase that is involved in cytoskeletal reorganization and apoptosis. SLK is a microtubule-associated protein inducing actin stress fiber disassembly, it also mediates apoptosis and it is ubiquitously expressed; highest expression is found in heart and in skeletal muscle. There are two named isoforms.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • CTCL tumor antigen se20 9 antibody
    • LOSK antibody
    • se20 9 antibody
    • Serine/threonine protein kinase 2 antibody
    • SLK antibody
    • SNF1 sucrose nonfermenting like kinase antibody
    • STE20 like serine/threonine-protein kinase antibody
    • STE20 related kinase antibody
    • STK2 antibody
    see all

Images

  • All lanes : Anti-SLK antibody (ab226986) at 1/1000 dilution

    Lane 1 : WT 293T cell extracts
    Lane 2 : SLK Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 143 kDa

  • 4% paraformaldehyde-fixed H1299 cells stained for SLK (green) using ab226986 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • All lanes : Anti-SLK antibody (ab226986) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) cell lysate
    Lane 2 : H1299 cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 143 kDa



    5% SDS-PAGE gel.

References

ab226986 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226986.
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