Key features and details
- Rabbit polyclonal to SLK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLK antibody
See all SLK primary antibodies
DescriptionRabbit polyclonal to SLK
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 1 and 50 of human SLK (NP_055535.2).
- MCF7, HeLa,and 293T whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 200 kDa (predicted molecular weight: 143 kDa).|
|IP||Use at 2 µg/mg of lysate.|
RelevanceSLK is a serine/threonine protein kinase that is involved in cytoskeletal reorganization and apoptosis. SLK is a microtubule-associated protein inducing actin stress fiber disassembly, it also mediates apoptosis and it is ubiquitously expressed; highest expression is found in heart and in skeletal muscle. There are two named isoforms.
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All lanes : Anti-SLK antibody (ab70230) at 1 µg/ml
Lane 1 : MCF7 whole cell lysate at 50 µg
Lane 2 : MCF7 whole cell lysate at 15 µg
Lane 3 : MCF7 whole cell lysate at 5 µg
Lane 4 : HeLa whole cell lysate at 15 µg
Lane 5 : 293T whole cell lysate at 15 µg
Predicted band size: 143 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
250µg MCF7 whole cell lysate immunoprecipitated with ab70230 at 3 µg/mg lysate and immunoblotted at 1µg/ml. The band at 50-60 kDa is due to the heavy chain of the antibody.
ab70230 has not yet been referenced specifically in any publications.