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Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)

Reviews (14)Q&A (8)References (84)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)
  • Western blot - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)

Key features and details

  • Mouse monoclonal [NOQ7.5.4D] to Slow Skeletal Myosin Heavy chain
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Rabbit
  • Isotype: IgG1

Overview

  • Product name

    Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D]
    See all Slow Skeletal Myosin Heavy chain primary antibodies

  • Description

    Mouse monoclonal [NOQ7.5.4D] to Slow Skeletal Myosin Heavy chain

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Rat, Rabbit
    Predicted to work with: Sheep, Goat, Chicken, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig

  • Immunogen

    Full length native protein (purified) corresponding to Human Slow Skeletal Myosin Heavy chain. Human skeletal muscle myosin purified from myofibrils.

  • Positive control

    • IHC-P: Rabbit tongue tissue. WB: Rat skeletal muscle tissue extract.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR201056, GR231200, GR285981. Lot numbers other than GR201056, GR231200, GR285981will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (5)
Use a concentration of 2.5 - 5 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
WB (1)
Use a concentration of 1.25 - 2.5 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 200 kDa).
Notes
IHC-P
Use a concentration of 2.5 - 5 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1.25 - 2.5 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 200 kDa).

Target

  • Function

    Muscle contraction.

  • Tissue specificity

    Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level).

  • Involvement in disease

    Defects in MYH7 are the cause of cardiomyopathy familial hypertrophic type 1 (CMH1) [MIM:192600]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in MYH7 are the cause of myopathy myosin storage (MYOMS) [MIM:608358]. In this disorder, muscle biopsy shows type 1 fiber predominance and increased interstitial fat and connective tissue. Inclusion bodies consisting of the beta cardiac myosin heavy chain are present in the majority of type 1 fibers, but not in type 2 fibers.
    Defects in MYH7 are the cause of scapuloperoneal myopathy MYH7-related (SPMM) [MIM:181430]; also known as scapuloperoneal syndrome myopathic type. SPMM is a progressive muscular atrophia beginning in the lower legs and affecting the shoulder region earlier and more severely than distal arm.
    Defects in MYH7 are a cause of cardiomyopathy dilated type 1S (CMD1S) [MIM:613426]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in MYH7 are the cause of myopathy distal type 1 (MPD1) [MIM:160500]. MPD1 is a muscular disorder characterized by early-onset selective weakness of the great toe and ankle dorsiflexors, followed by weakness of the finger extensors. Mild proximal weakness occasionally develops years later after the onset of the disease.

  • Sequence similarities

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.

  • Domain

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

  • Cellular localization

    Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt

  • Database links

    • Alternative names

      • Beta myosin heavy chain antibody
      • cardiac muscle beta isoform antibody
      • CMD1S antibody
      • CMH1 antibody
      • MPD1 antibody
      • MYH7 antibody
      • MYH7_HUMAN antibody
      • Myhc slow antibody
      • MyHC-beta antibody
      • MyHC-slow antibody
      • MYHCB antibody
      • Myopathy, distal 1 antibody
      • Myosin heavy chain (AA 1-96) antibody
      • Myosin heavy chain 7 antibody
      • Myosin heavy chain antibody
      • Myosin heavy chain slow isoform antibody
      • Myosin heavy chain, cardiac muscle beta isoform antibody
      • Myosin, heavy chain 7, cardiac muscle, beta antibody
      • Myosin, heavy polypeptide 7, cardiac muscle, beta antibody
      • Myosin-7 antibody
      • Rhabdomyosarcoma antigen MU RMS 40.7A antibody
      • SPMD antibody
      • SPMM antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded rabbit tomgue tissue staining Skeletal Myosin Heavy chain using ab11083 at 2.5 μg/mL.

    • Western blot - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)
      Western blot - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)
      Lane 1 : Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083) at 1.25 µg/ml
      Lane 2 : Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083) at 2.5 µg/ml
      Lane 3 : Negative control antibody

      All lanes : Rat skeletal muscle

      Secondary
      All lanes : Anti-Mouse IgG (Fac-specific)-peroxidase conjugate at 1/5000 dilution

      Predicted band size: 200 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)

      ab11083 at 1/500 dilution, staining Slow Skeletal Myosin Heavy chain in mouse tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)

      ab11083 at 1/4000 dilution, staining Slow Skeletal Myosin Heavy chain in rabbit tongue tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).

    References (84)

    Publishing research using ab11083? Please let us know so that we can cite the reference in this datasheet.

    ab11083 has been referenced in 84 publications.

    • Zhang XZ  et al. Procyanidins-crosslinked small intestine submucosa: A bladder patch promotes smooth muscle regeneration and bladder function restoration in a rabbit model. Bioact Mater 6:1827-1838 (2021). PubMed: 33336114
    • Hord JM  et al. Nox2 signaling and muscle fiber remodeling are attenuated by losartan administration during skeletal muscle unloading. Physiol Rep 9:e14606 (2021). PubMed: 33400850
    • Weng K  et al. Effects of marketable ages on meat quality through fiber characteristics in the goose. Poult Sci 100:728-737 (2021). PubMed: 33518126
    • Mouton AJ  et al. Interaction of Obesity and Hypertension on Cardiac Metabolic Remodeling and Survival Following Myocardial Infarction. J Am Heart Assoc 10:e018212 (2021). PubMed: 33666098
    • Chen X  et al. Quercetin regulates skeletal muscle fiber type switching via adiponectin signaling. Food Funct 12:2693-2702 (2021). PubMed: 33667291
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a question

    1-8 of 8 Q&A

    Question

    No western blot signal from mouse heart homogenates

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    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ****.

    To check the status of the order please contact our Customer Service team and reference this number.

    As I mentioned, optimizing the transfer buffer may encourage better transfer of higher molecular weight proteins like myosin. Addition of 0.1% SDS to the transfer buffer and reducing methanol to 5% or less are common approaches.

    I meant to ask how much sample you are loading per lane but 50ug should be sufficient. I am assuming you use protease inhibitors when you harvest and prepare the samples. If not, there may be some degradation of mysosin that impairs antibody recognition.

    Try ab97542 at 1/500 to start.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    Inquiry: Antibody Storage Conditions (temperature/reconstitution etc) Stored at -20C freezer Description of the Problem (high background, wrong band size, more bands, no bands etc) There is no band in western blot Sample (Species/Cell extract/Purified protein/Recombinant protein, etc) Samples performed include cell extract from rat cell culture, sheep diaphragm tissue and rat diaphragm tissue. The purified protein extract was used for the above samples. Sample Preparation (Buffer/Protease inhibitors/Heating sample etc.) Muscle samples were homogenized in ice-cold lysis buffer containing 20 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid pH 7.7, 2.5 mM MgCl2, 0.1 mM ethylenediaminetetraacetic acid, 20 mM b-glycerophosphate, 100 mM NaCl, 0.1% Triton 100, 500 uM dithiothreitol, 100 uMNa3Vo4, 100 mM phenylmethanesulfonylfluoride, 0.01%NP40 and protease inhibitor cocktail tablet (Roche, Castle Hill, Australia). Homogenates were subjected to six cycles of freeze–thaw. The supernatant was centrifuged at 10 000g for 25 min at 4C and total protein were collected. The cell pellet were performed using the same protocol without homogenizing process. Amount of protein loaded 100 ug protein were loaded in a 17 well pre-made gel. Electrophoresis/Gel Conditions (Reducing or non-reducing gel, % of the gel etc.) We used 12% 17 well SDS-PAGE Gel (NXG01227) with the running buffer from Expedon. The gel is run for 1.5h at 130v at room temperature. Transfer and blocking conditions (Buffer/time period, Blocking agent etc) Transfer o/n at 4C, 5% skim milk blocking for 1hr Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, wash step) Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083), used 1:5000 – 1:2000 dilution, incubated o/n, followed by three time wash. Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, wash step) Anti mouse lgG HRP-linked Antibody (cell Signaling, #7076), used 1:2000 dilution for 1hr, followed by 3 time wash. Detection Method (ECL, ECL Plus etc.) ECL Plus Positive and negative controls used (please specify) The membranes were reused after failing to obtain positive band from MHC slow antibody (ab11083). It was re-probed with MHC fast antibody (Novocastra Laboratories Ltd, NCL-MHCf). The positive result (band) was detected. OPTIMIZATION ATTEMPTS (PROBLEM SOLVING) How many times have you tried the Western Blot? Three times Have you run a “No Primary” control? No (Delete one) Do you obtain the same results every time? Yes (Delete one) e.g. are the background bands always in the same place? No band What steps have you altered? I changed MHC antibody concentration from 1:5000 dilutions to 1:2000 overnight incubation, but still no band detected.

    Read More
    Answer

    Thank you for providing the questionnaire. I am sorry that the customer is experiencing problems with this antibody. In order to see whether the vial is faulty, can you please check the following points with the customer:

    1.) I agree that the slow myosin heavy chain isoform should be expressed in human diaphragm. Can you confirm, that it is also expressed in rat diaphragm?

    2.) Can you confirm the secondary antibody is working, e.g. with another primary mouse antibody?

    3.) Maybe it would be worthwhile to try also another blocking buffer, such as BSA. Changing the blocking buffer can significantly improve the results as illustrated on the image of the datasheet of ab9385 for example https://www.abcam.com/HRP-GAPDH-antibody-Loading-Control-ab9385.html (or use the following: https://www.abcam.com/HRP-GAPDH-antibody-Loading-Control-ab9385.html).

    Do you have perhaps also our order number on which we have shipped this antibody? Indeed, the PO you send can be consolidated on another PO for shipping reasons.

    As you know, all our products are guaranteed by our Abpromise. Should the vial the customer received be faulty, we would be happy to replace or credit it.

    Thank you very much for your cooperation and help. I look forward to hear back from you.

    Read More

    Question

    Yes, I centrifuged all of the contents to the bottom of the tube but couldn’t get any more than 80ul out of the vial.

    The purchase order number was 5000 87689 and the order was placed on 6.6.12

    Thanks for your help with this matter

    Kind regards

    Read More
    Answer

    Thank you for confirming these details.

    I am sorry that the vial you received did not contain the full amount of the product and I apologize for the inconvenience. I have issued a free of charge replacement vial with the order number of ###.

    Please do not hesitate to contact us if you need anything further. Good luck with your future research.

    Read More

    Question

    Dear Sir/Madam,

    I recently purchased an antibody from your company (mouse monoclonal slow skeletal myosin heavy chain, ab11083), which specifies that there should be a 100ul volume in the vial, however when aliquotting the vial this morning I was disappointed to find only 80ul in the vial, which as I am sure you can appreciate is a considerable shortage (20%).

    Is there a way in which this can be resolved?

    Kind regards

    Read More
    Answer

    Thank you for contacting us. I am sorry that the vial you received did not contain the full amount of the product.

    It is important to centrifuge any vial containing liquid upon receipt. Could you confirm if the vial has been spun down?

    We recommend centrifuging for 10,000 x g for 20 seconds to pull down the solution that might be trapped in the threads of the vial and under the lid. For smaller vials please place them within a microcentrifuge tube first before centrifuging.

    Should this not provide the correct amount of product, I will be pleased to provide an immediate free of charge replacement vial. In order to arrange this, please contact me with the order number and date of purchase.

    Thank you for your cooperation. Please do not hesitate to contact us if you need anything further.

    Read More

    Question

    Product code: 51263

    Inquiry: 1) Order details: ˙Batch number (Lot number): gr25963-2 Po: 1069725 ˙Abcam product code: ab51263 ˙Antibody storage conditions (temperature/reconstitution etc) : -20℃
    2) Please describe the problem (high background, non-specific signal…etc). It can work with ab11083, but non-specific signal with ab51263 (same condition and same muscle).
    3) On what material are you testing the antibody in IHC/ICC? ˙Species: mice (C57/BL6) ˙What’s cell line or tissue: skeletal muscle
    4) Sample preparation: ˙Type of sample: formalin fixed ˙paraffin embedded sections, cells in culture, other:____) : paraffin embedded sections ˙Sample preparation: ˙Positive control : ˙Negative control :
    5) Fixation step ˙Yes or No : Yes ˙If yes, Fixative agent and concentration: 10 % neutral buffer formalin ˙Fixation time: ˙Fixation temperature: room temperature
    6) Antigen retrieval method(including time, temperature etc.): heat 15 mins
    7) Permeabilization method: ˙Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers? ˙Permeabilizing agent and concentration:
    8) Blocking agent (eg BSA, serum…): ˙Concentration: 3% ˙Blocking time :2 hr ˙Blocking temperature: room temperature
    9) ˙Endogenous peroxidases blocked? ˙Endogenous biotins blocked?
    10) Primary antibody (If more than one was used, describe in “additional notes”) : ˙Species: mice ˙Reacts against: ˙At what dilution(s) have you tested this antibody:1:100 and 1:150 ˙Diluent buffer: 1% BSA ˙Incubation time: overnight ˙Incubation temperature: 4℃ ˙What washing steps were done (which buffer, number of washes): TBST, 3×10 mins
    11) Secondary antibody: supersensitivy polymer-HRP IHC detecting system, QD4200810(Lot number) ˙Species: ˙Reacts against which species: ˙At what dilution(s) have you tested this antibody: ˙Diluent buffer: ˙Incubation time: ˙What washing steps were done (which buffer, number of washes): ˙Fluorochrome or enzyme conjugate (eg: FITC, HRP, AP, biotin…etc): ˙Do you know whether the problems you are experiencing come from the secondary?
    12) Signal amplification method (eg: ABC, LSAB, HRP polymer, TSE): HRP polymer
    13) Detection method (eg: DAB, BCIP/NBT …etc):DAB
    14) ˙ How many times have you run this staining? 3 times ˙Do you obtain the same results every time? YES ˙What steps have you altered to try and optimize the use of this antibody? antibody dilution(s)¸1:150 to 1:100

    Read More
    Answer

    Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

    Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab51263 Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32]. I would also appreciate if you can confirm some further details:

    1. Please keep in mind that you cannot compare two different antibodies against two different targets with each other. Every antibody needs its individual optimization.

    2. What was the HIER buffer? Did the customer perform a time series to find the optimal point? I would strongly recommend to perform a time series of at least 3 different retrieval times (like 15, 20, 25 min) to find the optimal time. As every antibody is different, every epitope may show a different retrieval time. The occurrence ofno signalusually shows a too short retrieval.

    3. Isthe customer using a detergent like Tween or Triton during incubation? If not, I can recommend adding Triton to your incubation solution to facilitate the antibodies solubility, but in a low concentration of 0.025%.

    Should the suggestions not improve the results, please do let me know.

    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    Read More

    Question

    Could you tell us the results.
    It's been a long time since last question. Thanks

    Read More
    Answer

    Apologies for the delay, I was waiting for further information.

    Our products team has confirmed that these products have the correct alternative names listed. As stated on the datasheets they will specifically stain either the fast or slow fibers.

    I hope this information is useful.

    Read More

    Question

    What is the recommended antigen retrieval protocol for ab7784 and ab11083? What secondary antibody was used to avoid detecting endogenous mouse IgG?

    Read More
    Answer

    I have obtained the following protocol information: Regarding ab7784 - Antigen retrieval is not necessary for this product (although we have found heat mediated treatment does enhance staining). No special pretreatment is necessary for staining of FFPE tissues. We would suggest the customer use a mouse on mouse kit if they wish to use this product on mouse tissue, but no particular kit is recommended. Regarding ab11083 - I have received the quality control methods used for this antibody, which suggest that a digestion with 0.1% pronase (also known as protease from Streptomyces griseus) for 8-10 minutes can be used. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

    Read More

    Question

    We would like to order anti myosin skeletal heavy chain antibodies against IIa and 2x chain.

    Read More
    Answer

    Thank you for contacting us. We have following products available in catalogue that are best match as per your request. ab11083 ab51263 ab104128 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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