Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Diaphragm)
Permeabilization
Yes - TBS/BSA/azide/Triton
Specification
Diaphragm
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Unfixed
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide/Triton
Dilution
1/1500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
This section was mistakenly not fixed (I assumed that the sections I was given were of fixed, snap-frozen muscle). However, in muscle MHC is in such abundance that it is easily immunodetected without fixation. Staining of nuclei ( with Hoechst) is diminished because of lack of fixation. Slow fibres are red stained with ab11083 (Fast fibres are green stained with ab91506).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
Submitted Jun 13 2022