Recombinant Anti-SLP-2 antibody [EPR18718] (ab191883)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18718] to SLP-2
- Suitable for: Flow Cyt (Intra), IP, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SLP-2 antibody [EPR18718]
See all SLP-2 primary antibodies -
Description
Rabbit monoclonal [EPR18718] to SLP-2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Jurkat, MCF7, A549, C6, RAW 264.7 and NIH/3T3 whole cell lysates; Human fetal kidney lysate; Mouse brain and spleen lysates; Rat brain lysate. ICC/IF: HeLa and Jurkat cells. Flow Cyt (intra): HeLa cells. IP: Jurkat whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18718 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/120.
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IP |
1/50.
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WB |
1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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ICC/IF |
1/250.
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Notes |
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Flow Cyt (Intra)
1/120. |
IP
1/50. |
WB
1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
ICC/IF
1/250. |
Target
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Tissue specificity
Ubiquitously expressed at low levels. -
Sequence similarities
Belongs to the band 7/mec-2 family. -
Cellular localization
Membrane. Cytoplasm. Cytoplasm > cytoskeleton. Associated with the cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 30968 Human
- Entrez Gene: 66592 Mouse
- Entrez Gene: 298203 Rat
- Omim: 608292 Human
- SwissProt: Q9UJZ1 Human
- SwissProt: Q99JB2 Mouse
- SwissProt: Q4FZT0 Rat
- Unigene: 3439 Human
see all -
Alternative names
- EPB72 like protein 2 antibody
- EPB72-like protein 2 antibody
- HSPC108 antibody
see all
Images
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Anti-SLP-2 antibody [EPR18718] (ab191883) at 1/1000 dilution + Human fetal kidney lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 39 kDa
Exposure time: 1 minuteBlocking/dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-SLP-2 antibody [EPR18718] (ab191883) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : A549 (Human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 30 secondsBlocking/dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-SLP-2 antibody [EPR18718] (ab191883) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse spleen lysate
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : Rat brain lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDaBlocking/dilution buffer: 5% NFDM/TBST.
Exposure time: Lane1,2,3 and 4:3 minutes;Lane 5 and 6:1 minute.
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Immunofluorescence staining of HeLa cells with ab191883 at a working dilution of 1 in 250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1 in 1000 (shown in green). The mitochondria were stained with ab33985 (mouse monoclonal to COX-IV, mitochondrial marker) at 1/1000 and ab150120 at 1/1000 (shown in red). The cells were fixed in 100% methanol and permeabilized using 0.1% triton-X 100. The negative controls are shown in bottom panels. For negative control 1, ab191883 was used at a dilution of 1/250 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/1000. For negative control 2, ab7291 (mouse monoclonal to alpha tubulin) was used at a dilution of 1/1000 followed by ab150077 (Alexa Fluor® 488 goat anti-rabbit) at a dilution of 1/1000.
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Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling SLP-2 with ab191883 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin -Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab191883 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution. -
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Jurkat cells (Human T cell leukemia cell line from peripheral blood) labeling SLP-2 with ab191883 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin -Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab191883 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution. -
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling SLP-2 with ab191883 at 1/120 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.
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SLP-2 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with ab191883 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab191883 at 1/1000 dilution. Veriblot for IP (HRP) (ab13136) was used as secondary antibody at 1/10000 dilution.
Lane 1: Jurkat whole cell lysate 10µg (Input).
Lane 2: ab191883 IP in Jurkat whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] (ab172730) instead of ab191883 in Jurkat whole cell cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab191883 has been referenced in 1 publication.
- Gonzalez-Velandia KY et al. Expression pattern of Stomatin-domain proteins in the peripheral olfactory system. Sci Rep 12:11447 (2022). PubMed: 35794236