Overview

  • Product name

  • Description

    Rabbit polyclonal to SLP76
  • Host species

    Rabbit
  • Specificity

    ab196599 detects endogenous level of total SLP76 protein
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to Human SLP76 (C terminal).
    Database link: Q13094

  • Positive control

    • U937, BT-474 cell extracts, mouse spleen and liver extracts; HeLa cells; mouse spleen tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, 49% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab196599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 60 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Involved in T-cell antigen receptor mediated signaling.
  • Tissue specificity

    Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
  • Sequence similarities

    Contains 1 SAM (sterile alpha motif) domain.
    Contains 1 SH2 domain.
  • Domain

    The SH2 domain mediates interaction with SHB.
  • Post-translational
    modifications

    Phosphorylated after T-cell receptor activation by ZAP-70.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 76 kDa tyrosine phosphoprotein antibody
    • CG8697 antibody
    • LCP 2 antibody
    • LCP2 antibody
    • LCP2_HUMAN antibody
    • Lymphocyte cytosolic protein 2 antibody
    • SH2 domain containing leukocyte protein 76 KD antibody
    • SH2 domain containing leukocyte protein of 76kD antibody
    • SH2 domain containing leukocyte protein of 76kDa antibody
    • SH2 domain-containing leukocyte protein of 76 kDa antibody
    • SLP 76 antibody
    • SLP 76 tyrosine phosphoprotein antibody
    • SLP-76 antibody
    • SLP-76 tyrosine phosphoprotein antibody
    • SLP76 antibody
    • SLP76 tyrosine phosphoprotein antibody
    see all

Images

  • All lanes : Anti-SLP76 antibody (ab196599) at 1/500 dilution

    Lane 1 : U937 cell extract
    Lane 2 : BT-474 cell extract
    Lane 3 : Mouse spleen extract
    Lane 4 : Mouse liver extract

    Predicted band size: 60 kDa

  • Immunofluoresence analysis of HeLa cells labeling SLP76 with ab196599 at 1/50 dilution. Blue: DAPI for nuclear staining.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling SLP76 with ab196599 at 1/100 dilution.

References

ab196599 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab196599.
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