Key features and details
- Rabbit polyclonal to SLP76
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SLP76 antibody
See all SLP76 primary antibodies
DescriptionRabbit polyclonal to SLP76
Specificityab196599 detects endogenous level of total SLP76 protein
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant fragment corresponding to Human SLP76 (C terminal).
Database link: Q13094
- U937, BT-474 cell extracts, mouse spleen and liver extracts; HeLa cells; mouse spleen tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 60 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionInvolved in T-cell antigen receptor mediated signaling.
Tissue specificityHighly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
Sequence similaritiesContains 1 SAM (sterile alpha motif) domain.
Contains 1 SH2 domain.
DomainThe SH2 domain mediates interaction with SHB.
modificationsPhosphorylated after T-cell receptor activation by ZAP-70.
- Information by UniProt
- 76 kDa tyrosine phosphoprotein antibody
- CG8697 antibody
- LCP 2 antibody
All lanes : Anti-SLP76 antibody (ab196599) at 1/500 dilution
Lane 1 : U937 cell extract
Lane 2 : BT-474 cell extract
Lane 3 : Mouse spleen extract
Lane 4 : Mouse liver extract
Predicted band size: 60 kDa
Immunofluoresence analysis of HeLa cells labeling SLP76 with ab196599 at 1/50 dilution. Blue: DAPI for nuclear staining.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling SLP76 with ab196599 at 1/100 dilution.
ab196599 has been referenced in 2 publications.
- Bunaciu RP et al. Retinoic acid and 6-formylindolo(3,2-b)carbazole (FICZ) combination therapy reveals putative targets for enhancing response in non-APL AML. Leuk Lymphoma 60:1697-1708 (2019). PubMed: 30570341
- Zhang Y et al. Transcriptome Landscape of Human Folliculogenesis Reveals Oocyte and Granulosa Cell Interactions. Mol Cell 72:1021-1034.e4 (2018). PubMed: 30472193