Key features and details
- Rabbit polyclonal to SLP76
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLP76 antibody
See all SLP76 primary antibodies
DescriptionRabbit polyclonal to SLP76
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human SLP76 aa 200-250. The exact sequence is proprietary.
Database link: Q13094
- WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226382 was affinity purified using an epitope specific to SLP76 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 60 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionInvolved in T-cell antigen receptor mediated signaling.
Tissue specificityHighly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
Sequence similaritiesContains 1 SAM (sterile alpha motif) domain.
Contains 1 SH2 domain.
DomainThe SH2 domain mediates interaction with SHB.
modificationsPhosphorylated after T-cell receptor activation by ZAP-70.
- Information by UniProt
- 76 kDa tyrosine phosphoprotein antibody
- CG8697 antibody
- LCP 2 antibody
All lanes : Anti-SLP76 antibody (ab226382) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
SLP76 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226382 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226382 at 1 µg/ml.
Lane 1: ab226382 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226382 has not yet been referenced specifically in any publications.