Overview

  • Product name

  • Description

    Rabbit polyclonal to SLP76
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human SLP76 aa 200-250. The exact sequence is proprietary.
    Database link: Q13094

  • Positive control

    • WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226382 was affinity purified using an epitope specific to SLP76 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Involved in T-cell antigen receptor mediated signaling.
  • Tissue specificity

    Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
  • Sequence similarities

    Contains 1 SAM (sterile alpha motif) domain.
    Contains 1 SH2 domain.
  • Domain

    The SH2 domain mediates interaction with SHB.
  • Post-translational
    modifications

    Phosphorylated after T-cell receptor activation by ZAP-70.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 76 kDa tyrosine phosphoprotein antibody
    • CG8697 antibody
    • LCP 2 antibody
    • LCP2 antibody
    • LCP2_HUMAN antibody
    • Lymphocyte cytosolic protein 2 antibody
    • SH2 domain containing leukocyte protein 76 KD antibody
    • SH2 domain containing leukocyte protein of 76kD antibody
    • SH2 domain containing leukocyte protein of 76kDa antibody
    • SH2 domain-containing leukocyte protein of 76 kDa antibody
    • SLP 76 antibody
    • SLP 76 tyrosine phosphoprotein antibody
    • SLP-76 antibody
    • SLP-76 tyrosine phosphoprotein antibody
    • SLP76 antibody
    • SLP76 tyrosine phosphoprotein antibody
    see all

Images

  • All lanes : Anti-SLP76 antibody (ab226382) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 60 kDa


    Exposure time: 30 seconds


    Lysates prepared using NETN lysis buffer.

  • SLP76 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226382 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226382 at 1 µg/ml.

    Lane 1: ab226382 IP in Jurkat whole cell lysate.

    Lane 2: Control IgG IP in Jurkat whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 30 seconds.

References

ab226382 has not yet been referenced specifically in any publications.

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