Recombinant Anti-SLP76 antibody [EPR2549(2)] (ab109254)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2549(2)] to SLP76
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SLP76 antibody [EPR2549(2)]
See all SLP76 primary antibodies -
Description
Rabbit monoclonal [EPR2549(2)] to SLP76 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MOLT4 or HuT78 cell lysates; Human tonsil tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2549(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Involved in T-cell antigen receptor mediated signaling. -
Tissue specificity
Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines. -
Sequence similarities
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH2 domain. -
Domain
The SH2 domain mediates interaction with SHB. -
Post-translational
modificationsPhosphorylated after T-cell receptor activation by ZAP-70. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3937 Human
- Entrez Gene: 16822 Mouse
- Entrez Gene: 155918 Rat
- Omim: 601603 Human
- SwissProt: Q13094 Human
- SwissProt: Q60787 Mouse
- Unigene: 304475 Human
- Unigene: 265350 Mouse
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Alternative names
- 76 kDa tyrosine phosphoprotein antibody
- CG8697 antibody
- LCP 2 antibody
see all
Images
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ab109254 at 1/100 dilution, staining SLP76 in Human tonsil, by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab109254 showing positive staining in Normal human spleen tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-SLP76 antibody [EPR2549(2)] (ab109254) at 1/1000 dilution
Lane 1 : HuT-78 cell lysate
Lane 2 : MOLT-4 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa -
ab109254 showing positive staining in Normal human colon tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab109254 showing negative staining in Normal human brain tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab109254 showing negative staining in Normal human heart tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab109254 has been referenced in 1 publication.
- Hoshino A et al. Dysregulation of Epstein-Barr Virus Infection in Hypomorphic ZAP70 Mutation. J Infect Dis 218:825-834 (2018). PubMed: 29684201