Recombinant
RabMAb

Anti-SLP76 antibody [EPR2549(2)] (ab109254)

Overview

  • Product name
    Anti-SLP76 antibody [EPR2549(2)]
    See all SLP76 primary antibodies
  • Description
    Rabbit monoclonal [EPR2549(2)] to SLP76
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SLP76 aa 500-600 (C terminal). The exact sequence is proprietary.

  • Positive control
    • MOLT4 or HuT78 cell lysates; Human tonsil tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR2549(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Involved in T-cell antigen receptor mediated signaling.
    • Tissue specificity
      Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
    • Sequence similarities
      Contains 1 SAM (sterile alpha motif) domain.
      Contains 1 SH2 domain.
    • Domain
      The SH2 domain mediates interaction with SHB.
    • Post-translational
      modifications
      Phosphorylated after T-cell receptor activation by ZAP-70.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 76 kDa tyrosine phosphoprotein antibody
      • CG8697 antibody
      • LCP 2 antibody
      • LCP2 antibody
      • LCP2_HUMAN antibody
      • Lymphocyte cytosolic protein 2 antibody
      • SH2 domain containing leukocyte protein 76 KD antibody
      • SH2 domain containing leukocyte protein of 76kD antibody
      • SH2 domain containing leukocyte protein of 76kDa antibody
      • SH2 domain-containing leukocyte protein of 76 kDa antibody
      • SLP 76 antibody
      • SLP 76 tyrosine phosphoprotein antibody
      • SLP-76 antibody
      • SLP-76 tyrosine phosphoprotein antibody
      • SLP76 antibody
      • SLP76 tyrosine phosphoprotein antibody
      see all

    Images

    • ab109254 at 1/100 dilution, staining SLP76 in Human tonsil, by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109254 showing positive staining in Normal spleen tissue.

    • All lanes : Anti-SLP76 antibody [EPR2549(2)] (ab109254) at 1/1000 dilution

      Lane 1 : HuT78 cell lysate
      Lane 2 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 60 kDa

    • ab109254 showing positive staining in Normal colon tissue.

    • ab109254 showing negative staining in Normal brain tissue.

    • ab109254 showing negative staining in Normal heart tissue.

    References

    ab109254 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109254.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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