Recombinant
RabMAb

Recombinant Anti-SLP76 antibody [EPR2549(2)] (ab109254)

Overview

  • Product name

    Anti-SLP76 antibody [EPR2549(2)]
    See all SLP76 primary antibodies
  • Description

    Rabbit monoclonal [EPR2549(2)] to SLP76
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SLP76 aa 500-600 (C terminal). The exact sequence is proprietary.

  • Positive control

    • MOLT4 or HuT78 cell lysates; Human tonsil tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2549(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Involved in T-cell antigen receptor mediated signaling.
    • Tissue specificity

      Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
    • Sequence similarities

      Contains 1 SAM (sterile alpha motif) domain.
      Contains 1 SH2 domain.
    • Domain

      The SH2 domain mediates interaction with SHB.
    • Post-translational
      modifications

      Phosphorylated after T-cell receptor activation by ZAP-70.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • 76 kDa tyrosine phosphoprotein antibody
      • CG8697 antibody
      • LCP 2 antibody
      • LCP2 antibody
      • LCP2_HUMAN antibody
      • Lymphocyte cytosolic protein 2 antibody
      • SH2 domain containing leukocyte protein 76 KD antibody
      • SH2 domain containing leukocyte protein of 76kD antibody
      • SH2 domain containing leukocyte protein of 76kDa antibody
      • SH2 domain-containing leukocyte protein of 76 kDa antibody
      • SLP 76 antibody
      • SLP 76 tyrosine phosphoprotein antibody
      • SLP-76 antibody
      • SLP-76 tyrosine phosphoprotein antibody
      • SLP76 antibody
      • SLP76 tyrosine phosphoprotein antibody
      see all

    Images

    • ab109254 at 1/100 dilution, staining SLP76 in Human tonsil, by Immunohistochemistry, Paraffin-embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab109254 showing positive staining in Normal human spleen tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • All lanes : Anti-SLP76 antibody [EPR2549(2)] (ab109254) at 1/1000 dilution

      Lane 1 : HuT78 cell lysate
      Lane 2 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 60 kDa

    • ab109254 showing positive staining in Normal human colon tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab109254 showing negative staining in Normal human brain tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab109254 showing negative staining in Normal human heart tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab109254 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109254.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up