Recombinant
RabMAb

Recombinant Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990)

Overview

  • Product name

    Anti-SLP76 (phospho Y113) antibody [EPR2851]
    See all SLP76 primary antibodies
  • Description

    Rabbit monoclonal [EPR2851] to SLP76 (phospho Y113)
  • Host species

    Rabbit
  • Specificity

    ab75990 detects SLP76 phosphorylated on tyrosine 113
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Rabbit, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding tyrosine 113 of human SLP76.

  • Positive control

    • Jurkat cytoplasmic lysate treated with pervanadate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2851
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa).
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Involved in T-cell antigen receptor mediated signaling.
    • Tissue specificity

      Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
    • Sequence similarities

      Contains 1 SAM (sterile alpha motif) domain.
      Contains 1 SH2 domain.
    • Domain

      The SH2 domain mediates interaction with SHB.
    • Post-translational
      modifications

      Phosphorylated after T-cell receptor activation by ZAP-70.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • 76 kDa tyrosine phosphoprotein antibody
      • CG8697 antibody
      • LCP 2 antibody
      • LCP2 antibody
      • LCP2_HUMAN antibody
      • Lymphocyte cytosolic protein 2 antibody
      • SH2 domain containing leukocyte protein 76 KD antibody
      • SH2 domain containing leukocyte protein of 76kD antibody
      • SH2 domain containing leukocyte protein of 76kDa antibody
      • SH2 domain-containing leukocyte protein of 76 kDa antibody
      • SLP 76 antibody
      • SLP 76 tyrosine phosphoprotein antibody
      • SLP-76 antibody
      • SLP-76 tyrosine phosphoprotein antibody
      • SLP76 antibody
      • SLP76 tyrosine phosphoprotein antibody
      see all

    Images

    • All lanes : Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate - untreated
      Lane 2 : Jurkat cell lysate - treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 60 kDa
      Observed band size: 76 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Cimo AM  et al. CD25 and CD69 induction by a4ß1 outside-in signalling requires TCR early signalling complex proteins. Biochem J 454:109-21 (2013). WB ; Human . Read more (PubMed: 23758320) »
    • Köhler K  et al. Matched sizes of activating and inhibitory receptor/ligand pairs are required for optimal signal integration by human natural killer cells. PLoS One 5:e15374 (2010). WB ; Human . Read more (PubMed: 21179506) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    1e+006 cells
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Rabbit Cell lysate - whole cell (T cells)
    Specification
    T cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Dr. Youg Thaker

    Verified customer

    Submitted May 15 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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