Recombinant
RabMAb

Recombinant Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (ab206782)

Overview

  • Product name

    Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free
    See all SLP76 primary antibodies
  • Description

    Rabbit monoclonal [EP2853Y] to SLP76 (phospho Y145) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody detects SLP76 phosphorylated on tyrosine 145.

  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cyt, Dot blotmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SLP76 aa 100-200 (phospho Y145). The exact sequence is proprietary.
    Database link: Q13094

  • Positive control

    • Jurkat cell lysates treated with pervanadate.
  • General notes

    ab206782 is the carrier-free version of ab75829 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab206782 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EP2853Y
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab206782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
Flow Cyt Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Involved in T-cell antigen receptor mediated signaling.
    • Tissue specificity

      Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
    • Sequence similarities

      Contains 1 SAM (sterile alpha motif) domain.
      Contains 1 SH2 domain.
    • Domain

      The SH2 domain mediates interaction with SHB.
    • Post-translational
      modifications

      Phosphorylated after T-cell receptor activation by ZAP-70.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • 76 kDa tyrosine phosphoprotein antibody
      • CG8697 antibody
      • LCP 2 antibody
      • LCP2 antibody
      • LCP2_HUMAN antibody
      • Lymphocyte cytosolic protein 2 antibody
      • SH2 domain containing leukocyte protein 76 KD antibody
      • SH2 domain containing leukocyte protein of 76kD antibody
      • SH2 domain containing leukocyte protein of 76kDa antibody
      • SH2 domain-containing leukocyte protein of 76 kDa antibody
      • SLP 76 antibody
      • SLP 76 tyrosine phosphoprotein antibody
      • SLP-76 antibody
      • SLP-76 tyrosine phosphoprotein antibody
      • SLP76 antibody
      • SLP76 tyrosine phosphoprotein antibody
      see all

    References

    ab206782 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab206782.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up