• Product name

  • Description

    Rabbit polyclonal to SLPI
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human SLPI aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab129950)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human Lung; Human Spleen; Human Small Intestine; Human Colon. It also gave a positive signal in HeLa, Hek293 and HepG2 cells in IF.



Our Abpromise guarantee covers the use of ab82337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/250. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).


  • Function

    Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues.
  • Tissue specificity

    Mucous fluids.
  • Sequence similarities

    Contains 2 WAP domains.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • ALK 1 antibody
    • ALK1 antibody
    • ALP antibody
    • Antileukoprotease antibody
    • Antileukoproteinase 1 antibody
    • Antileukoproteinase antibody
    • Antileukoproteinase1 antibody
    • BLPI antibody
    • Human seminal protease inhibitor antibody
    • HUSI 1 antibody
    • HUSI antibody
    • HUSI I antibody
    • HUSI-1 antibody
    • MPI antibody
    • Mucus proteinase inhibitor antibody
    • Protease inhibitor WAP4 antibody
    • Secretory leukocyte peptidase inhibitor antibody
    • Secretory leukocyte protease inhibitor antibody
    • Seminal proteinase inhibitor antibody
    • SLPI antibody
    • SLPI_HUMAN antibody
    • WAP four disulfide core domain protein 4 antibody
    • WAP four-disulfide core domain protein 4 antibody
    • WAP4 antibody
    • WFDC4 antibody
    see all


  • All lanes : Anti-SLPI antibody (ab82337) at 1/250 dilution

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 4 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa
    Additional bands at: 29 kDa, 48 kDa, 8 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 20 minutes
  • ICC/IF image of ab82337 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82337, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) Hek293 and HepG2 cells at 5µg/ml.


ab82337 has not yet been referenced specifically in any publications.

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