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  1. Link

    slpi-antibody-aminoterminal-end-ab46763.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-SLPI antibody - Aminoterminal end (ab46763)

  • Datasheet
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Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763)
  • Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763)

Key features and details

  • Rabbit polyclonal to SLPI - Aminoterminal end
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SLPI antibody - Aminoterminal end
    See all SLPI primary antibodies
  • Description

    Rabbit polyclonal to SLPI - Aminoterminal end
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide based on the aminoterminal end of human SLPI.(Please note: the amino acid sequence is proprietary) (Peptide available as ab46833.)

  • Positive control

    • WB: Recombinant Human SLP I, Wild-type HeLa cell lysate, PC-3 cell lysate, LNCaP cell lysate
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Adaptive Immunity
    • B Cells
    • Non-CD
    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Other protease inhibitors
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human SLPI protein (ab99142)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab46763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 14 kDa.

A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection.

Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 14 kDa.

A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection.

Target

  • Function

    Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues.
  • Tissue specificity

    Mucous fluids.
  • Sequence similarities

    Contains 2 WAP domains.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P03973 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6590 Human
    • GenBank: NM_003064 Human
    • Omim: 107285 Human
    • SwissProt: P03973 Human
    • Unigene: 517070 Human
    • Alternative names

      • ALK 1 antibody
      • ALK1 antibody
      • ALP antibody
      • Antileukoprotease antibody
      • Antileukoproteinase 1 antibody
      • Antileukoproteinase antibody
      • Antileukoproteinase1 antibody
      • BLPI antibody
      • Human seminal protease inhibitor antibody
      • HUSI 1 antibody
      • HUSI antibody
      • HUSI I antibody
      • HUSI-1 antibody
      • MPI antibody
      • Mucus proteinase inhibitor antibody
      • Protease inhibitor WAP4 antibody
      • Secretory leukocyte peptidase inhibitor antibody
      • Secretory leukocyte protease inhibitor antibody
      • Seminal proteinase inhibitor antibody
      • SLPI antibody
      • SLPI_HUMAN antibody
      • WAP four disulfide core domain protein 4 antibody
      • WAP four-disulfide core domain protein 4 antibody
      • WAP4 antibody
      • WFDC4 antibody
      see all

    Images

    • Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763)
      Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763)
      All lanes : Anti-SLPI antibody - Aminoterminal end (ab46763) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : SLPI knockout HeLa cell lysate
      Lane 3 : PC-3 cell lysate
      Lane 4 : LNCaP cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 14 kDa
      Observed band size: 22 kDa why is the actual band size different from the predicted?



      False colour image of Western blot: Anti-SLPI antibody - Aminoterminal end staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab46763 was shown to bind specifically to SLPI. A band was observed at 22 kDa in wild-type HeLa cell lysates with no signal observed at this size in SLPI knockout cell line ab265215 (knockout cell lysate ab257685). To generate this image, wild-type and SLPI knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye®800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

    • Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763)
      Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763)
      All lanes : Anti-SLPI antibody - Aminoterminal end (ab46763) at 1/1000 dilution

      Lane 1 : Recombinant Human SLP I at 0.04 µg
      Lane 2 : Recombinant Human SLP I at 0.02 µg
      Lane 3 : Recombinant Human SLP I at 0.01 µg
      Lane 4 : Recombinant Human SLP I at 0.004 µg
      Lane 5 : Recombinant Human SLP I at 0.001 µg

      Predicted band size: 14 kDa
      Observed band size: 15 kDa why is the actual band size different from the predicted?

    Protocols

    • Recommended WB protocol

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (2)

    Publishing research using ab46763? Please let us know so that we can cite the reference in this datasheet.

    ab46763 has been referenced in 2 publications.

    • Klimenkova O  et al. A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation. Blood 123:1239-49 (2014). Human . PubMed: 24352879
    • Yang Y  et al. Quantitative proteomic analysis of oral brush biopsies identifies secretory leukocyte protease inhibitor as a promising, mechanism-based oral cancer biomarker. PLoS One 9:e95389 (2014). WB ; Human . PubMed: 24748380

    Customer reviews and Q&As

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