Anti-SLPI antibody - Aminoterminal end (ab46763)
- Datasheet
- References (2)
- Protocols
Overview
-
Product nameAnti-SLPI antibody - Aminoterminal end
See all SLPI primary antibodies -
DescriptionRabbit polyclonal to SLPI - Aminoterminal end
-
Host speciesRabbit
-
Tested applicationsSuitable for: WBmore details
-
Species reactivityReacts with: Human
-
Immunogen
Synthetic peptide based on the aminoterminal end of human SLPI.(Please note: the amino acid sequence is proprietary) (Peptide available as ab46833.)
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab46763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 14 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. |
Target
-
FunctionAcid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues.
-
Tissue specificityMucous fluids.
-
Sequence similaritiesContains 2 WAP domains.
-
Cellular localizationSecreted.
- Information by UniProt
-
Database links
- Entrez Gene: 6590 Human
- GenBank: NM_003064 Human
- Omim: 107285 Human
- SwissProt: P03973 Human
- Unigene: 517070 Human
-
Alternative names
- ALK 1 antibody
- ALK1 antibody
- ALP antibody
see all
Images
-
Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763)All lanes : Anti-SLPI antibody - Aminoterminal end (ab46763) at 1/1000 dilution
Lane 1 : Recombinant Human SLP I at 0.04 µg
Lane 2 : Recombinant Human SLP I at 0.02 µg
Lane 3 : Recombinant Human SLP I at 0.01 µg
Lane 4 : Recombinant Human SLP I at 0.004 µg
Lane 5 : Recombinant Human SLP I at 0.001 µg
Predicted band size: 14 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
This product has been referenced in:
- Klimenkova O et al. A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation. Blood 123:1239-49 (2014). Human . Read more (PubMed: 24352879) »
- Yang Y et al. Quantitative proteomic analysis of oral brush biopsies identifies secretory leukocyte protease inhibitor as a promising, mechanism-based oral cancer biomarker. PLoS One 9:e95389 (2014). WB ; Human . Read more (PubMed: 24748380) »
