Anti-SLPI antibody - Aminoterminal end (ab46763)
Key features and details
- Rabbit polyclonal to SLPI - Aminoterminal end
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLPI antibody - Aminoterminal end
See all SLPI primary antibodies -
Description
Rabbit polyclonal to SLPI - Aminoterminal end -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide based on the aminoterminal end of human SLPI.(Please note: the amino acid sequence is proprietary) (Peptide available as ab46833.)
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Positive control
- WB: Recombinant Human SLP I, Wild-type HeLa cell lysate, PC-3 cell lysate, LNCaP cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab46763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 14 kDa.
A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. |
| Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 14 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. |
Target
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Function
Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues. -
Tissue specificity
Mucous fluids. -
Sequence similarities
Contains 2 WAP domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6590 Human
- GenBank: NM_003064 Human
- Omim: 107285 Human
- SwissProt: P03973 Human
- Unigene: 517070 Human
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Alternative names
- ALK 1 antibody
- ALK1 antibody
- ALP antibody
see all
Images
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Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763)All lanes : Anti-SLPI antibody - Aminoterminal end (ab46763) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SLPI knockout HeLa cell lysate
Lane 3 : PC-3 cell lysate
Lane 4 : LNCaP cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-SLPI antibody - Aminoterminal end staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab46763 was shown to bind specifically to SLPI. A band was observed at 22 kDa in wild-type HeLa cell lysates with no signal observed at this size in SLPI knockout cell line ab265215 (knockout cell lysate ab257685). To generate this image, wild-type and SLPI knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye®800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763)All lanes : Anti-SLPI antibody - Aminoterminal end (ab46763) at 1/1000 dilution
Lane 1 : Recombinant Human SLP I at 0.04 µg
Lane 2 : Recombinant Human SLP I at 0.02 µg
Lane 3 : Recombinant Human SLP I at 0.01 µg
Lane 4 : Recombinant Human SLP I at 0.004 µg
Lane 5 : Recombinant Human SLP I at 0.001 µg
Predicted band size: 14 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (2)
ab46763 has been referenced in 2 publications.
- Klimenkova O et al. A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation. Blood 123:1239-49 (2014). Human . PubMed: 24352879
- Yang Y et al. Quantitative proteomic analysis of oral brush biopsies identifies secretory leukocyte protease inhibitor as a promising, mechanism-based oral cancer biomarker. PLoS One 9:e95389 (2014). WB ; Human . PubMed: 24748380
