Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11111] to SLU7
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
Product nameAnti-SLU7 antibody [EPR11111]
See all SLU7 primary antibodies
DescriptionRabbit monoclonal [EPR11111] to SLU7
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SLU7 aa 500 to the C-terminus. The exact sequence is proprietary.
Database link: O95391
- HeLa, 293 and Jurkat cell lysates; 293 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa).|
|ICC/IF||1/100 - 1/250.|
FunctionParticipates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation.
Sequence similaritiesBelongs to the SLU7 family.
Contains 1 CCHC-type zinc finger.
DomainThe CCHC-type zinc finger is required to retain the protein within the nucleus and prevent its shuttle back to the cytoplasm via the CRM1 pathway.
Cellular localizationNucleus. Cytoplasm. Predominantly nuclear. Shuttling between the nucleus and the cytoplasm is regulated by the CCHC-type zinc finger. Upon UV-C stress stimulus, the nuclear concentration of the protein decreases, affecting alternative splicing.
- Information by UniProt
- 9G8 antibody
- hSlu7 antibody
- MGC9280 antibody
All lanes : Anti-SLU7 antibody [EPR11111] (ab181138) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 68 kDa
Anti-SLU7 antibody [EPR11111] (ab181138) at 1/10000 dilution + Jurkat cell lysate at 20 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 68 kDa
Immunofluorescent analysis of 293 cells (paraformaldehyde-fixed, 4%) labeling SLU7 with ab181138 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
ab181138 has not yet been referenced specifically in any publications.