Overview

  • Product name

    Anti-SLU7 antibody [EPR11111]
    See all SLU7 primary antibodies
  • Description

    Rabbit monoclonal [EPR11111] to SLU7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SLU7 aa 500 to the C-terminus. The exact sequence is proprietary.
    Database link: O95391

  • Positive control

    • HeLa, 293 and Jurkat cell lysates; 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa).
ICC/IF 1/100 - 1/250.

Target

  • Function

    Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation.
  • Sequence similarities

    Belongs to the SLU7 family.
    Contains 1 CCHC-type zinc finger.
  • Domain

    The CCHC-type zinc finger is required to retain the protein within the nucleus and prevent its shuttle back to the cytoplasm via the CRM1 pathway.
  • Cellular localization

    Nucleus. Cytoplasm. Predominantly nuclear. Shuttling between the nucleus and the cytoplasm is regulated by the CCHC-type zinc finger. Upon UV-C stress stimulus, the nuclear concentration of the protein decreases, affecting alternative splicing.
  • Information by UniProt
  • Database links

  • Alternative names

    • 9G8 antibody
    • hSlu7 antibody
    • MGC9280 antibody
    • Pre mRNA splicing factor SLU7 antibody
    • Pre-mRNA-splicing factor slu7 antibody
    • SLU 7 antibody
    • slu7 antibody
    • SLU7 splicing factor homolog (S. cerevisiae) antibody
    • SLU7 splicing factor homolog antibody
    • SLU7_HUMAN antibody
    • splicing factor antibody
    • Step II splicing factor SLU7 antibody
    • Zinc knuckle motif containing antibody
    see all

Images

  • All lanes : Anti-SLU7 antibody [EPR11111] (ab181138) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 68 kDa

  • Anti-SLU7 antibody [EPR11111] (ab181138) at 1/10000 dilution + Jurkat cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 68 kDa

  • Immunofluorescent analysis of 293 cells (paraformaldehyde-fixed, 4%) labeling SLU7 with ab181138 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

References

ab181138 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181138.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up