Key features and details
- Rabbit polyclonal to SLU7 (phospho S513 + S515)
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLU7 (phospho S513 + S515) antibody
See all SLU7 primary antibodies
DescriptionRabbit polyclonal to SLU7 (phospho S513 + S515)
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey
Synthetic peptide within Human SLU7 (C terminal) (phospho S513). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: O95391
- ICC/IF: HeLa and HCT 116 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
FunctionParticipates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation.
Sequence similaritiesBelongs to the SLU7 family.
Contains 1 CCHC-type zinc finger.
DomainThe CCHC-type zinc finger is required to retain the protein within the nucleus and prevent its shuttle back to the cytoplasm via the CRM1 pathway.
Cellular localizationNucleus. Cytoplasm. Predominantly nuclear. Shuttling between the nucleus and the cytoplasm is regulated by the CCHC-type zinc finger. Upon UV-C stress stimulus, the nuclear concentration of the protein decreases, affecting alternative splicing.
- Information by UniProt
- 9G8 antibody
- hSlu7 antibody
- MGC9280 antibody
4% paraformaldehyde-fixed HCT 116 (human colorectal carcinoma cell line) cells stained for SLU7 (phospho S513 + S515) (green) using ab227960 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SLU7 (phospho S513 + S515) (green) using ab227960 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue). Phalloidin, a cytoskeletal marker was stained at 1/200 dilution (red).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227960 has not yet been referenced specifically in any publications.