Overview

  • Product name

    Anti-SLU7 (phospho S513 + S515) antibody
    See all SLU7 primary antibodies
  • Description

    Rabbit polyclonal to SLU7 (phospho S513 + S515)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide within Human SLU7 (C terminal) (phospho S513). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: O95391

  • Positive control

    • ICC/IF: HeLa and HCT 116 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation.
  • Sequence similarities

    Belongs to the SLU7 family.
    Contains 1 CCHC-type zinc finger.
  • Domain

    The CCHC-type zinc finger is required to retain the protein within the nucleus and prevent its shuttle back to the cytoplasm via the CRM1 pathway.
  • Cellular localization

    Nucleus. Cytoplasm. Predominantly nuclear. Shuttling between the nucleus and the cytoplasm is regulated by the CCHC-type zinc finger. Upon UV-C stress stimulus, the nuclear concentration of the protein decreases, affecting alternative splicing.
  • Information by UniProt
  • Database links

  • Alternative names

    • 9G8 antibody
    • hSlu7 antibody
    • MGC9280 antibody
    • Pre mRNA splicing factor SLU7 antibody
    • Pre-mRNA-splicing factor slu7 antibody
    • SLU 7 antibody
    • slu7 antibody
    • SLU7 splicing factor homolog (S. cerevisiae) antibody
    • SLU7 splicing factor homolog antibody
    • SLU7_HUMAN antibody
    • splicing factor antibody
    • Step II splicing factor SLU7 antibody
    • Zinc knuckle motif containing antibody
    see all

Images

  • 4% paraformaldehyde-fixed HCT 116 (human colorectal carcinoma cell line) cells stained for SLU7 (phospho S513 + S515) (green) using ab227960 at 1/500 dilution in ICC/IF. 

    Nuclear counter stain: Hoechst 33342 (blue).

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SLU7 (phospho S513 + S515) (green) using ab227960 at 1/500 dilution in ICC/IF.

    Nuclear counter stain: Hoechst 33342 (blue). Phalloidin, a cytoskeletal marker was stained at 1/200 dilution (red).

     

References

ab227960 has not yet been referenced specifically in any publications.

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