This antibody gave a positive signal in MCF7 overexpressing SLUG whole cell lysate.
This antibody has only been tested on overexpressing cell lysates; we have no data on the detection of the endogenous protein.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa).
Transcriptional repressor. Involved in the generation and migration of neural crest cells.
Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
Involvement in disease
Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
Belongs to the snail C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers.
Slug (chicken homolog) zinc finger protein antibody
Slug homolog zinc finger protein antibody
Slug zinc finger protein antibody
SLUGH 1 antibody
SNAI 2 antibody
Snail 2 antibody
Snail homolog 2 antibody
WS 2D antibody
Zinc finger protein SLUG antibody
Zinc finger protein SNAI2 antibody
Western blot - Anti-SLUG antibody (ab106077)
All lanes : Anti-SLUG antibody (ab106077) at 1 µg/ml
Lane 1 : MCF7 cell lysate overexpressing SLUG protein Lane 2 : MCF7 cell lysate overexpressing SNAIL protein Lane 3 : MCF7 cell lysate transfected with vector control plasmid Lane 4 : Untransfected MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Immunohistochemistry (Frozen sections) - Anti-SLUG antibody (ab106077)This image is courtesy of an anonymous abreview.
ab106077 staining SLUG on an E9.5 mouse embryo section by immunohistochemistry. The tissue was paraformaldehyde fixed and permeabilized in PBST prior to blocking in NOVOCASTRA protein block for 30 minutes at 25°C. The primary antibody was diluted 1/25 and incubated with the sample for 48 hour at 4°C. An Alexa Fluor 555 conjugated donkey anti-rabbit antibody, diluted 1/1000, was used as the secondary. Nuclear staining shown with DAPI.