Product nameAnti-SLUG antibody
See all SLUG primary antibodies
DescriptionRabbit polyclonal to SLUG
Specificityab27568 detects the overexpressing human Slug protein, however we have no data on the detection of the endogenous Human protein with this current batch. ab27568 does not cross react with overexpressed SNAIL protein in cell lysates.
Tested applicationsSuitable for: WB, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Chicken
Predicted to work with: Cow, Dog, Human, Pig, Xenopus laevis
- This antibody gave a positive signal in the following tissue lysates: Mouse heart, Rat heart as well as MCF7 cell lysate overexpressing SLUG protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab27568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 30-34 kDa (predicted molecular weight: 30 kDa).|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 18716062|
FunctionTranscriptional repressor. Involved in the generation and migration of neural crest cells.
Tissue specificityExpressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
Involvement in diseaseDefects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
Sequence similaritiesBelongs to the snail C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.
- Information by UniProt
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
- Protein snail homolog 2 antibody
Transverse section showing Slug-expressing neural crest cells (green) in the dorsal neural tube (NT) in the hindbrain of a quail embryo. 4% PFA fixed embryonic day 1.7 Japanese Quail embryo, frozen in Tissutek, sectioned 12 um. Ab27568 1/400, incubated overnight. Goat anti-rabbit: Alexa488 (Molecular Probes) 1/1000 incubated for 2 hours.
All lanes : Anti-SLUG antibody (ab27568) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Heart (Rat) Tissue Lysate
Lysates/proteins at 30 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Secondary antibody - goat anti-rabbit HRP (preadsorbed)
All lanes : Anti-SLUG antibody (ab27568) at 1/1000 dilution
Lane 1 : MCF7 cell lysate overexpressing SLUG protein
Lane 2 : MCF7 lysate overexpressing SNAIL protein
Lane 3 : MCF7 lysate tranfected with vector control plasmid
All lanes : HRP-conjugated anti-rabbit antibody at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
ab27568 detects a band of 34 kDa in MCF7 cells that have been transfected with a vector that overexpresses the SLUG protein. This antibody does not crossreact with the SNAIL protein, as it did not recognize this protein in MCF7 cells overexpressing SNAIL. ab27568 was unable to detect endogenous SLUG in MDA-MB231 cells (not shown).
ab27568 staining mouse embryonic stem cells by ICC/IF. Cells were formaldehyde fixed and permeabilized in 0.1% Triton X-100 prior to blocking with 0.1% BSA and 1% serum for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated for 2 hours at 21°C. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody, diluted 1/500, was used as the secondary.
This product has been referenced in:
- Li L et al. MiR-101-3p inhibits EMT to attenuate proliferation and metastasis in glioblastoma by targeting TRIM44. J Neurooncol 141:19-30 (2019). Read more (PubMed: 30539341) »
- Feng S et al. Tripartite motif-containing 14 (TRIM14) promotes epithelial-mesenchymal transition via ZEB2 in glioblastoma cells. J Exp Clin Cancer Res 38:57 (2019). Read more (PubMed: 30728039) »