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Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human SLUG.
Our Abpromise guarantee covers the use of ab27568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 30-34 kDa (predicted molecular weight: 30 kDa).|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 18716062|
Secondary antibody - goat anti-rabbit HRP (preadsorbed)
ab27568 staining mouse embryonic stem cells by ICC/IF. Cells were formaldehyde fixed and permeabilized in 0.1% Triton X-100 prior to blocking with 0.1% BSA and 1% serum for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated for 2 hours at 21°C. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody, diluted 1/500, was used as the secondary.
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