Anti-SLUG antibody [mAbcam51772] (ab51772)
Key features and details
- Mouse monoclonal [mAbcam51772] to SLUG
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-SLUG antibody [mAbcam51772]
See all SLUG primary antibodies -
Description
Mouse monoclonal [mAbcam51772] to SLUG -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Cow, Pig, Xenopus laevis -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Mouse Skin Tissue Lysate
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
mAbcam51772 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (4) |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 30 kDa.
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Notes |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 30 kDa. |
Target
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Function
Transcriptional repressor. Involved in the generation and migration of neural crest cells. -
Tissue specificity
Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. -
Involvement in disease
Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. -
Sequence similarities
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 432368 Chicken
- Entrez Gene: 6591 Human
- Entrez Gene: 20583 Mouse
- Entrez Gene: 379440 Xenopus laevis
- Omim: 602150 Human
- SwissProt: Q3MHQ4 Cow
- SwissProt: O43623 Human
- SwissProt: P97469 Mouse
see all -
Alternative names
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
- Protein snail homolog 2 antibody
see all
Images
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All lanes : Anti-SLUG antibody [mAbcam51772] (ab51772)
Lane 1 : Untransfected MCF7 cells
Lane 2 : MCF7 cells transfected with SLUG
Lane 3 : MCF7 cells transfected with Snail
Predicted band size: 30 kDa
Observed band size: 30 kDa -
Anti-SLUG antibody [mAbcam51772] (ab51772) at 5 µg/ml +
Mouse skin tissue lysate - total protein (ab4025) at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 34 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes -
All lanes : Anti-SLUG antibody [mAbcam51772] (ab51772) at 1 µg/ml
Lane 1 : Skin tissue lysate from Slug heterozygous mouse
Lane 2 : Skin tissue lysate from Slug knockout mouse
Performed under reducing conditions.
Predicted band size: 30 kDa
Datasheets and documents
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Datasheet download
References (36)
ab51772 has been referenced in 36 publications.
- Chen X et al. Identification of hub genes predicting the development of prostate cancer from benign prostate hyperplasia and analyzing their clinical value in prostate cancer by bioinformatic analysis. Discov Oncol 13:54 (2022). PubMed: 35768705
- Xing X et al. No Effect on Tumorigenesis in MG63 Cells Induced by Co-Cultured Mesenchymal Stem Cells. J Oncol 2022:4202439 (2022). PubMed: 35847369
- Liu H et al. TAB2 Promotes the Biological Functions of Head and Neck Squamous Cell Carcinoma Cells via EMT and PI3K Pathway. Dis Markers 2022:1217918 (2022). PubMed: 35978886
- Zhou PL et al. Circular RNA hsa_circ_0000277 sequesters miR-4766-5p to upregulate LAMA1 and promote esophageal carcinoma progression. Cell Death Dis 12:676 (2021). PubMed: 34226522
- Liang X et al. Irradiation Activates MZF1 to Inhibit miR-541-5p Expression and Promote Epithelial-Mesenchymal Transition (EMT) in Radiation-Induced Pulmonary Fibrosis (RIPF) by Upregulating Slug. Int J Mol Sci 22:N/A (2021). PubMed: 34768749