Overview

  • Product name
    Anti-SLUG antibody [mAbcam51772]
    See all SLUG primary antibodies
  • Description
    Mouse monoclonal [mAbcam51772] to SLUG
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Cow, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human SLUG.

    Read Abcam's proprietary immunogen policy (Peptide available as ab27840.)

  • Positive control
    • Mouse Skin Tissue Lysate
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab51772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 24932263

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 30 kDa.
ICC/IF 1/100.
IHC-Fr 1/100.

Target

  • Function
    Transcriptional repressor. Involved in the generation and migration of neural crest cells.
  • Tissue specificity
    Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
  • Involvement in disease
    Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
  • Sequence similarities
    Belongs to the snail C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC10182 antibody
    • Neural crest transcription factor Slug antibody
    • Protein snail homolog 2 antibody
    • Slug (chicken homolog) zinc finger protein antibody
    • Slug homolog zinc finger protein antibody
    • Slug zinc finger protein antibody
    • SLUGH antibody
    • SLUGH 1 antibody
    • SLUGH1 antibody
    • SNAI 2 antibody
    • Snai2 antibody
    • SNAI2_HUMAN antibody
    • Snail 2 antibody
    • Snail homolog 2 antibody
    • Snail2 antibody
    • WS 2D antibody
    • WS2D antibody
    • Zinc finger protein SLUG antibody
    • Zinc finger protein SNAI2 antibody
    see all

Images

  • All lanes : Anti-SLUG antibody [mAbcam51772] (ab51772)

    Lane 1 : Untransfected MCF7 cells
    Lane 2 : MCF7 cells transfected with SLUG
    Lane 3 : MCF7 cells transfected with Snail

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Anti-SLUG antibody [mAbcam51772] (ab51772) at 5 µg/ml + Mouse skin tissue lysate - total protein (ab4025) at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa
    Additional bands at: 34 kDa, 60 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 minutes
  • All lanes : Anti-SLUG antibody [mAbcam51772] (ab51772) at 1 µg/ml

    Lane 1 : Skin tissue lysate from Slug heterozygous mouse
    Lane 2 : Skin tissue lysate from Slug knockout mouse

    Performed under reducing conditions.

    Predicted band size: 30 kDa

  • ab51772 staining SLUG in mouse mammary gland cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with PF4% Triton 0,5% for 5min and blocking with 10% serum for 1 hour at RT was performed. Samples were incubated with primary antibody (1/100) for 1 hour. An Alexa Fluor®488-conjugated goat polyclonal to mouse IgG (H+L) was used at dilution at 1/750 as secondary antibody. Blue colour in the image represents DAPI staining.

    See Abreview

References

This product has been referenced in:
  • Shi B  et al. MiR-106a directly targets LIMK1 to inhibit proliferation and EMT of oral carcinoma cells. Cell Mol Biol Lett 24:1 (2019). Read more (PubMed: 30873211) »
  • Zhang H  et al. Loss of profilin 2 contributes to enhanced epithelial-mesenchymal transition and metastasis of colorectal cancer. Int J Oncol 53:1118-1128 (2018). Read more (PubMed: 30015842) »
See all 7 Publications for this product

Customer reviews and Q&As

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1-9 of 9 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (breast cancer tissue, tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6
Permeabilization
Yes - tween 20
Specification
breast cancer tissue, tonsil
Blocking step
ab diluent as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 07 2017

Application
Western blot
Sample
Human Tissue lysate - whole (Human prostate cancer cell)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Specification
Human prostate cancer cell
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 31 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
Human Cell (endothelial cell)
Specification
endothelial cell
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 29 2014

Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jan 23 2013

Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Dr. Ruxandra Sirbulescu

Verified customer

Submitted Nov 14 2011

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Ms. Mayssaa NASSOUR

Verified customer

Submitted Jul 27 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse mammary gland)
Specification
mouse mammary gland
Fixative
Paraformaldehyde
Permeabilization
Yes - PF4% triton 0,5% 5min
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Ms. Mayssaa NASSOUR

Verified customer

Submitted Jul 22 2009

Application
Western blot
Sample
Mouse Tissue lysate - whole (skin, comma D ß cell line)
Loading amount
40 µg
Specification
skin, comma D ß cell line
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Ms. Mayssaa NASSOUR

Verified customer

Submitted Jul 21 2009

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa 293T)
Loading amount
30 µg
Specification
HeLa 293T
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Antibody Solutions

Verified customer

Submitted Sep 03 2008

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