• Product name

    Anti-SLURP1 antibody
  • Description

    Rabbit polyclonal to SLURP1
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human SLURP1 aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P55000

  • Positive control

    • This antibody gave a positive signal in WB within Human Skin tissue lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal skin.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab93840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Relevance

    SLURP1 (Secreted LY6/PLAUR domain containing 1) belongs to the Ly6/uPAR family but lacks a GPI-anchoring signal sequence. SLURP1 potentiates alpha-7 nicotinic acetylcholine receptors (CHRNA7) in keratinocytes and is implicated in maintaining the physiological and structural integrity of the keratinocyte layers of the skin. It is a marker of late differentiation of the skin and has an antitumor activity. Mutations in the SLURP1 gene are the cause of Mal de Meleda (MDM), a rare autosomal recessive genetic disease, characterized by inflammatory palmoplantar keratoderma.
  • Cellular localization

  • Database links

  • Alternative names

    • Anti neoplastic urinary protein antibody
    • ANUP antibody
    • ARS antibody
    • ARS component B antibody
    • ARS(component B) 81/S antibody
    • ArsB antibody
    • LY6LS antibody
    • Lymphocyte antigen 6 like secreted antibody
    • MDM antibody
    • Results in cobblestone changes in the skin of the palm antibody
    • Secreted Ly 6/uPAR related protein 1 antibody
    • Secreted LY6/PLAUR domain containing 1 antibody
    • Secreted Ly6/uPAR related protein 1 antibody
    • SLURP 1 antibody
    see all


  • IHC image of SLURP1 staining in Human normal skin formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93840, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-SLURP1 antibody (ab93840) at 1 µg/ml + Human skin tissue lysate - total protein (ab30166) at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 9 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 45 kDa (possible non-specific binding), 58 kDa (possible non-specific binding), 95 kDa (possible non-specific binding)

    Exposure time: 8 minutes

    The band observed at 9 kDa could potentially be a cleaved form of SLURP1 due to the presence of a 22 amino acid signal peptide.

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab93840 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406


ab93840 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab93840.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact partnerships@abcam.com

Sign up