Overview

  • Product name
    Anti-Sm-D2 antibody [EPR16762]
    See all Sm-D2 primary antibodies
  • Description
    Rabbit monoclonal [EPR16762] to Sm-D2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human Sm-D2 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P62316

  • Positive control
    • WB: HepG2, MCF7, A549, HeLa, Mouse brain, Mouse spleen, Rat brain, Mouse spleen, C6, RAW 264.7 and PC12 lysates. IHC: Human and Rat kidney tissues. ICC/IF: HeLa and MCF7 cells. FC: HeLa cells
  • General notes

     

     

    Previously labelled as SNRPD2. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR16762
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab198296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
ICC/IF 1/300.
IHC-P 1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/150.
IP Use at an assay dependent concentration.

Target

  • Function
    Required for pre-mRNA splicing. Required for snRNP biogenesis.
  • Sequence similarities
    Belongs to the snRNP core protein family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Sm-D2 antibody
    • small nuclear ribonucleoprotein D2 antibody
    • Small nuclear ribonucleoprotein D2 (RCG54604) antibody
    • small nuclear ribonucleoprotein D2 polypeptide (16.5kD) antibody
    • small nuclear ribonucleoprotein D2 polypeptide 16.5kDa antibody
    • SMALL NUCLEAR RIBONUCLEOPROTEIN POLYPEPTIDE D2; SNRPD2 antibody
    • Small nuclear ribonucleoprotein Sm D2 antibody
    • SMD2 antibody
    • SMD2_HUMAN antibody
    • snRNP core protein D2 antibody
    • SNRPD1 antibody
    • Snrpd2 antibody
    • SNRPD2 small nuclear ribonucleoprotein D2 polypeptide 16.5kDa antibody
    see all

Images

  • All lanes : Anti-Sm-D2 antibody [EPR16762] (ab198296) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Sm-D2 with ab198296 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nucleus staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

    Negative control Using PBS instead of primary antbody, secondary ab is Goat Anti-Rabbit IgG H&L (HRP)

  • Lanes 1-6 : Anti-Sm-D2 antibody [EPR16762] (ab198296) at 1/2000 dilution
    Lane 7 : Anti-Sm-D2 antibody [EPR16762] (ab198296) at 1/20000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : C6 cell lysate
    Lane 6 : RAW 264.7 cell lysate
    Lane 7 : PC12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Sm-D2 with ab198296 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nucleus staining on Rat kidney tissue is observed. Counter stained with Hematoxylin.

    Negative control Using PBS instead of primary antbody, secondary ab is Goat Anti-Rabbit IgG H&L (HRP)

  • NRPD2 protein was immunoprecipitation from 1mg of MCF-7 (Human breast adenocarcinoma) whole cell lysate with ab198296 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab198296 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution. Lane 1: Input, MCF-7 (Human breast adenocarcinoma) whole cell lysate, 10ug. Lane 2: IP of NRPD2 from MCF-7 (Human breast adenocarcinoma) whole cell lysate. Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab198296 in MCF-7 (Human breast adenocarcinoma) whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Flow cytometry analysis of HeLa cells labelling Sm-D2 (red) with purified ab198296 at dilution of 1/150. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

References

ab198296 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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