Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (ab212857)
Key features and details
- Mouse monoclonal [TAGLN/247] to TAGLN/Transgelin - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free
See all TAGLN/Transgelin primary antibodies -
Description
Mouse monoclonal [TAGLN/247] to TAGLN/Transgelin - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Pig -
Immunogen
Recombinant full length protein corresponding to Human TAGLN/Transgelin aa 1 to the C-terminus.
Database link: Q01995 -
Positive control
- Human colon carcinoma tissue; U-2 OS, Hs68 and WI 38 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab212857 is purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
TAGLN/247 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 1 - 2 µg/ml.
|
|
Flow Cyt |
Use 0.5-1µg for 106 cells.
(in 0.1 ml). |
|
IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT) |
Notes |
---|
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
Flow Cyt
Use 0.5-1µg for 106 cells. (in 0.1 ml). |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (Primary incubation for 30 minutes at RT) |
Target
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Function
Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. -
Sequence similarities
Belongs to the calponin family.
Contains 1 calponin-like repeat.
Contains 1 CH (calponin-homology) domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6876 Human
- Entrez Gene: 21345 Mouse
- Entrez Gene: 25123 Rat
- SwissProt: Q01995 Human
- SwissProt: P37804 Mouse
- SwissProt: P31232 Rat
- Unigene: 410977 Human
- Unigene: 283283 Mouse
see all -
Alternative names
- 22 kDa actin-binding protein antibody
- Protein WS3-10 antibody
- SM22 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab212857 has been referenced in 5 publications.
- Xu Z et al. Effects of shRNA-mediated silencing of PDE5A3 on intracellular cGMP and free Ca2+ levels and human prostate smooth muscle cell proliferation from benign prostatic hyperplasia. Exp Ther Med 21:322 (2021). PubMed: 33732295
- Chen R et al. CHOP Increases TRIB3-Dependent miR-208 Expression to Potentiate Vascular Smooth Muscle Cell Proliferation and Migration by Downregulating TIMP3 in Atherosclerosis. Cardiovasc Drugs Ther N/A:N/A (2021). PubMed: 33856595
- Xu (徐兴丽) X et al. Knockout of the NONO Gene Inhibits Neointima Formation in a Mouse Model of Vascular Injury. Arterioscler Thromb Vasc Biol 41:1428-1445 (2021). PubMed: 33626912
- Huang C et al. Drug-Eluting Stent Targeting Sp-1-Attenuated Restenosis by Engaging YAP-Mediated Vascular Smooth Muscle Cell Phenotypic Modulation. J Am Heart Assoc 9:e014103 (2020). PubMed: 31880978
- Lino M et al. Diabetic Vascular Calcification Mediated by the Collagen Receptor Discoidin Domain Receptor 1 via the Phosphoinositide 3-Kinase/Akt/Runt-Related Transcription Factor 2 Signaling Axis. Arterioscler Thromb Vasc Biol 38:1878-1889 (2018). PubMed: 29930002