Product nameAnti-Smac / Diablo antibody [EPR10934]
See all Smac / Diablo primary antibodies
DescriptionRabbit monoclonal [EPR10934] to Smac / Diablo
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human Smac/ Diablo. The exact sequence is proprietary.
Database link: Q9NR28
- HeLa, MCF7, Jurkat, 293T and Human testis lysates; Human heart and testis tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 27 kDa.|
FunctionPromotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
Tissue specificityUbiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
DomainThe mature N-terminus mediates interaction with XIAP.
Cellular localizationMitochondrion. Released into the cytosol when cells undergo apoptosis.
- Information by UniProt
- 0610041G12Rik antibody
- DBLOH_HUMAN antibody
- DBOH antibody
Immunohistochemical analysis of paraffinn embedded Human testis tissue labeling Smac / Diablo with ab174284 at 1/50.
Immunohistochemical analysis of paraffinn embedded Human heart tissue labeling Smac / Diablo with ab174284 at 1/50.
All lanes : Anti-Smac / Diablo antibody [EPR10934] (ab174284) at 1/1000 dilution
Lane 1 : Hela cell line lysate
Lane 2 : MCF7 cell line lysate
Lane 3 : Jurkat cell line lysate
Lane 4 : 293T cell line lysate
Lane 5 : Human testis tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
ab174284 has not yet been referenced specifically in any publications.