Flow Cytometry abreview for Anti-Smac / Diablo antibody [Y12]

Average
Abreviews
Application
Flow Cytometry
Sample
Human Cell (Platelets)
Specification
Platelets
Preparation
Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy
Platelets

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Flour488 Chicken Anti Rabbit IgG (H+L)
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Abcam response
Thank you for your valuable feedback. Reviewing the details, we notice the secondary alone shows quite a lot of flouresence in this case. To reduce this, we would suggest to try reducing the concentration of secondary antibody. This would hopefully decrease any non specific staining from the secondary.

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Submitted Dec 31 2010

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