Western blot abreview for Anti-Smac / Diablo antibody [Y12]

Excellent
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15% Gel)
Sample
Rat Tissue lysate - other (Skeletal muscle cytosolic fractions)
Specification
Skeletal muscle cytosolic fractions
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 3% milk
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ECL+
Bands
Specific: 21 kDa
Exposure
1 minute(s) and 40 second(s)

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Submitted Feb 19 2015

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