Recombinant
RabMAb

Anti-Smac / Diablo antibody [EPR10934] (ab174284)

Overview

  • Product name
    Anti-Smac / Diablo antibody [EPR10934]
    See all Smac / Diablo primary antibodies
  • Description
    Rabbit monoclonal [EPR10934] to Smac / Diablo
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Smac/ Diablo. The exact sequence is proprietary.
    Database link: Q9NR28

  • Positive control
    • HeLa, MCF7, Jurkat, 293T and Human testis lysates; Human heart and testis tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 27 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
    • Tissue specificity
      Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
    • Domain
      The mature N-terminus mediates interaction with XIAP.
    • Cellular localization
      Mitochondrion. Released into the cytosol when cells undergo apoptosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • 0610041G12Rik antibody
      • DBLOH_HUMAN antibody
      • DBOH antibody
      • DFNA64 antibody
      • diablo antibody
      • Diablo homolog (Drosophila) antibody
      • Diablo homolog antibody
      • Diablo homolog mitochondrial antibody
      • Diablo IAP binding mitochondrial protein antibody
      • Diablo like protein antibody
      • DIABLO S antibody
      • Direct IAP binding protein with low pI antibody
      • Direct IAP-binding protein with low pI antibody
      • FLJ10537 antibody
      • FLJ25049 antibody
      • mitochondrial antibody
      • Mitochondrial Smac protein antibody
      • Second mitochondria derived activator of caspase antibody
      • Second mitochondria-derived activator of caspase antibody
      • SMAC 3 antibody
      • Smac antibody
      • Smac protein antibody
      • SMAC3 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffinn embedded Human testis tissue labeling Smac / Diablo with ab174284 at 1/50.

    • Immunohistochemical analysis of paraffinn embedded Human heart tissue labeling Smac / Diablo with ab174284 at 1/50.

    • All lanes : Anti-Smac / Diablo antibody [EPR10934] (ab174284) at 1/1000 dilution

      Lane 1 : Hela cell line lysate
      Lane 2 : MCF7 cell line lysate
      Lane 3 : Jurkat cell line lysate
      Lane 4 : 293T cell line lysate
      Lane 5 : Human testis tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/500 dilution

      Developed using the ECL technique.

      Predicted band size: 27 kDa

    References

    ab174284 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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