Recombinant Anti-Smac/Diablo antibody [EPR10934] (ab174284)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac / Diablo antibody [EPR10934] (ab174284)](https://www.abcam.com/ps/products/174/ab174284/Images/ab174284-199512-ab174284IHC2.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac / Diablo antibody [EPR10934] (ab174284)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit Monoclonal [EPR10934] to Smac/Diablo
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Smac/Diablo antibody [EPR10934]
See all Smac/Diablo primary antibodies -
Description
Rabbit Monoclonal [EPR10934] to Smac/Diablo -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human Smac/ Diablo. The exact sequence is proprietary.
Database link: Q9NR28 -
Positive control
- HeLa, MCF7, Jurkat, 293T and Human testis lysates; Human heart and testis tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10934 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab174284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 27 kDa. |
Target
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Function
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). -
Tissue specificity
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. -
Domain
The mature N-terminus mediates interaction with XIAP. -
Cellular localization
Mitochondrion. Released into the cytosol when cells undergo apoptosis. - Information by UniProt
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Database links
- Entrez Gene: 56616 Human
- Entrez Gene: 66593 Mouse
- Entrez Gene: 288753 Rat
- Omim: 605219 Human
- SwissProt: Q9NR28 Human
- SwissProt: Q9JIQ3 Mouse
- Unigene: 169611 Human
- Unigene: 46716 Mouse
see all -
Alternative names
- 0610041G12Rik antibody
- DBLOH_HUMAN antibody
- DBOH antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac / Diablo antibody [EPR10934] (ab174284)
Immunohistochemical analysis of paraffinn embedded Human testis tissue labeling Smac / Diablo with ab174284 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac / Diablo antibody [EPR10934] (ab174284)](https://www.abcam.com/ps/products/174/ab174284/Images/ab174284-199510-ab174284IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac / Diablo antibody [EPR10934] (ab174284)Immunohistochemical analysis of paraffinn embedded Human heart tissue labeling Smac / Diablo with ab174284 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Smac / Diablo antibody [EPR10934] (ab174284)](https://www.abcam.com/ps/products/174/ab174284/Images/ab174284-199508-ab174284WB.jpg)
Western blot - Anti-Smac / Diablo antibody [EPR10934] (ab174284)All lanes : Anti-Smac/Diablo antibody [EPR10934] (ab174284) at 1/1000 dilution
Lane 1 : Hela cell line lysate
Lane 2 : MCF7 cell line lysate
Lane 3 : Jurkat cell line lysate
Lane 4 : 293T cell line lysate
Lane 5 : Human testis tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa -
![Anti-Smac / Diablo antibody [EPR10934] (ab174284)](https://www.abcam.com/ps/products/174/ab174284/Images/ab174284-4-benefits-of-recombinant-antibodies.png)
Anti-Smac / Diablo antibody [EPR10934] (ab174284)
Protocols
Datasheets and documents
References (0)
ab174284 has not yet been referenced specifically in any publications.
