Recombinant Anti-Smac/Diablo antibody [EPR10934] (ab174284)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Smac/Diablo antibody [EPR10934]
See all Smac/Diablo primary antibodies -
Description
Rabbit Monoclonal [EPR10934] to Smac/Diablo -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human Smac/ Diablo. The exact sequence is proprietary.
Database link: Q9NR28 -
Positive control
- HeLa, MCF7, Jurkat, 293T and Human testis lysates; Human heart and testis tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10934 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab174284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 27 kDa. |
Target
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Function
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). -
Tissue specificity
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. -
Domain
The mature N-terminus mediates interaction with XIAP. -
Cellular localization
Mitochondrion. Released into the cytosol when cells undergo apoptosis. - Information by UniProt
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Database links
- Entrez Gene: 56616 Human
- Entrez Gene: 66593 Mouse
- Entrez Gene: 288753 Rat
- Omim: 605219 Human
- SwissProt: Q9NR28 Human
- SwissProt: Q9JIQ3 Mouse
- Unigene: 169611 Human
- Unigene: 46716 Mouse
see all -
Alternative names
- 0610041G12Rik antibody
- DBLOH_HUMAN antibody
- DBOH antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac / Diablo antibody [EPR10934] (ab174284)](https://www.abcam.com/ps/products/174/ab174284/Images/ab174284-199512-ab174284IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac / Diablo antibody [EPR10934] (ab174284)Immunohistochemical analysis of paraffinn embedded Human testis tissue labeling Smac / Diablo with ab174284 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac / Diablo antibody [EPR10934] (ab174284)](https://www.abcam.com/ps/products/174/ab174284/Images/ab174284-199510-ab174284IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac / Diablo antibody [EPR10934] (ab174284)Immunohistochemical analysis of paraffinn embedded Human heart tissue labeling Smac / Diablo with ab174284 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Smac / Diablo antibody [EPR10934] (ab174284)](https://www.abcam.com/ps/products/174/ab174284/Images/ab174284-199508-ab174284WB.jpg)
Western blot - Anti-Smac / Diablo antibody [EPR10934] (ab174284)All lanes : Anti-Smac/Diablo antibody [EPR10934] (ab174284) at 1/1000 dilution
Lane 1 : Hela cell line lysate
Lane 2 : MCF7 cell line lysate
Lane 3 : Jurkat cell line lysate
Lane 4 : 293T cell line lysate
Lane 5 : Human testis tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Datasheets and documents
References
ab174284 has not yet been referenced specifically in any publications.
