Recombinant Anti-Smac/Diablo antibody [Y12] - BSA and Azide free (ab239797)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit Monoclonal [Y12] to Smac/Diablo - BSA and Azide free
- Suitable for: IP, IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Smac/Diablo antibody [Y12] - BSA and Azide free
See all Smac/Diablo primary antibodies -
Description
Rabbit Monoclonal [Y12] to Smac/Diablo - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Smac/ Diablo (C terminal). The exact sequence is proprietary.
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Epitope
ab239797 reacts with an epitope located in the C terminal region of Smac / Diablo. -
General notes
Ab239797 is the carrier-free version of ab32023. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab239797 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y12 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab239797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP | Use at an assay dependent concentration. | |
IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
ICC/IF | Use at an assay dependent concentration. | |
WB | Use at an assay dependent concentration. Detects a band of approximately 21 kDa (predicted molecular weight: 27 kDa). |
Target
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Function
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). -
Tissue specificity
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. -
Domain
The mature N-terminus mediates interaction with XIAP. -
Cellular localization
Mitochondrion. Released into the cytosol when cells undergo apoptosis. - Information by UniProt
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Database links
- Entrez Gene: 56616 Human
- Entrez Gene: 66593 Mouse
- Entrez Gene: 288753 Rat
- Omim: 605219 Human
- SwissProt: Q9NR28 Human
- SwissProt: Q9JIQ3 Mouse
- Unigene: 169611 Human
- Unigene: 46716 Mouse
see all -
Alternative names
- 0610041G12Rik antibody
- DBLOH_HUMAN antibody
- DBOH antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Smac / Diablo antibody [Y12] - BSA and Azide free (ab239797)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Smac / Diablo with ab32023 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32023).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac / Diablo antibody [Y12] - BSA and Azide free (ab239797)
ab32023, at a 1/10 dilution, staining human Smac/Diablo in skin cancer tissue by Immunohistochemistry, Paraffin embedded tissue
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32023).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-Smac / Diablo antibody [Y12] - BSA and Azide free (ab239797)Image from Lamers F et al. BMC Cancer. 2012 Jul 12;12:285. doi: 10.1186/1471-2407-12-285. Fig 3.; 12 July 2012 BMC Cancer 2012 12:285.
Immunofluorescence analysis of IMR32 cells, staining Smac / Diablo with ab32023. Cells were fixed with 4% paraformaldehyde before incubating with primary antibody. An Alexa Fluor® conjugated anti-rabbit IgG was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32023).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab239797 has not yet been referenced specifically in any publications.