Recombinant
RabMAb

Recombinant Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] - BSA and Azide free (ab223072)

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Overview

  • Product name

    Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] - BSA and Azide free
    See all SMAD 1 + SMAD9 primary antibodies

  • Description

    Rabbit monoclonal [EPR2290(2)] to SMAD 1 + SMAD9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human SMAD 1 + SMAD9.

  • Positive control

    • 293T, A673, Human heart and HeLa lysates

  • General notes

    Ab223072 is the carrier-free version of ab108965. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab223072 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab223072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    • Function

      Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.

    • Tissue specificity

      Ubiquitous. Highest expression seen in the heart and skeletal muscle.

    • Sequence similarities

      Belongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.

    • Post-translational
      modifications

      Phosphorylated on serine by BMP type 1 receptor kinase.
      Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.

    • Cellular localization

      Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • BSP-1 antibody
      • BSP1 antibody
      • HsMAD1 antibody
      • JV4-1 antibody
      • MAD homolog 1 antibody
      • Mad-related protein 1 antibody
      • MADH1 antibody
      • MADR1 antibody
      • Mothers against decapentaplegic homolog 1 antibody
      • Mothers against DPP homolog 1 antibody
      • SMAD 1 antibody
      • SMAD family member 1 antibody
      • Smad1 antibody
      • SMAD1_HUMAN antibody
      • Transforming growth factor-beta-signaling protein 1 antibody
      see all

    Images

    • Western blot - Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] - BSA and Azide free (ab223072)
      Western blot - Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] - BSA and Azide free (ab223072)
      All lanes : Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] (ab108965) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : A673 lysate
      Lane 3 : Human heart lysate
      Lane 4 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1 & 3-4 : HRP labelled goat anti-rabbit at 1/2000 dilution


      Predicted band size: 52 kDa
      Observed band size: 58 kDa
      why is the actual band size different from the predicted?

    References

    ab223072 has not yet been referenced specifically in any publications.

    Publishing research using ab223072? Please let us know so that we can cite the reference in this datasheet.

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