
Recombinant Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] - BSA and Azide free (ab223072)
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Overview
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Product name
Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] - BSA and Azide free
See all SMAD 1 + SMAD9 primary antibodies -
Description
Rabbit monoclonal [EPR2290(2)] to SMAD 1 + SMAD9 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to residues in Human SMAD 1 + SMAD9.
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Positive control
- 293T, A673, Human heart and HeLa lysates
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General notes
Ab223072 is the carrier-free version of ab108965. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab223072 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2290(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab223072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa). |
Target
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Function
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. -
Tissue specificity
Ubiquitous. Highest expression seen in the heart and skeletal muscle. -
Sequence similarities
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
Post-translational
modificationsPhosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4086 Human
- Entrez Gene: 17125 Mouse
- Entrez Gene: 25671 Rat
- Omim: 601595 Human
- SwissProt: Q15797 Human
- SwissProt: P70340 Mouse
- SwissProt: P97588 Rat
- Unigene: 604588 Human
see all -
Alternative names
- BSP-1 antibody
- BSP1 antibody
- HsMAD1 antibody
see all
Images
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All lanes : Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] (ab108965) at 1/1000 dilution
Lane 1 : 293T lysate
Lane 2 : A673 lysate
Lane 3 : Human heart lysate
Lane 4 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1 & 3-4 : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Datasheets and documents
References
ab223072 has not yet been referenced specifically in any publications.