• Product name

    Anti-SMAD 1/5/8/9 antibody
  • Description

    Rabbit polyclonal to SMAD 1/5/8/9
  • Host species

  • Specificity

    ab13723 may cross-react with SMAD2 or SMAD3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 18-32 and amino acids 315-329 of Human SMAD1. These sequences are identical in human SMAD5 and SMAD8/9.

  • Positive control

    • A549 whole cell lysate.



Our Abpromise guarantee covers the use of ab13723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 51 kDa.


  • Relevance

    SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs, common-mediator SMAD and inhibitory SMADs. SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology. SMAD8 and SMAD9 are used as alternate names for the same protein. Human SMAD1 is a 465 amino acid protein, human SMAD5 is a 465 amino acid and human SMAD9 is a 430 amino acid protein.
  • Cellular localization

  • Database links

  • Alternative names

    • DPC4 antibody
    • Madh6 antibody
    • MADH9 antibody
    • MADR1 antibody
    • SMAD family member 1 antibody
    • SMAD family member 5 antibody
    • SMAD family member 9 antibody
    • SMAD8A antibody
    • SMAD8B antibody
    see all


  • Western blot analysis of SMAD1/5/8/9 in 15 ugs of A549 cell lysate using ab13723 at 0.5 ug/ml.

    Western blot analysis of SMAD1/5/8/9 in 15 ugs of A549 cell lysate using ab13723 at 0.5 ug/ml.


This product has been referenced in:

  • Gao Y  et al. Propofol inhibits pancreatic cancer progress under hypoxia via ADAM8. J Hepatobiliary Pancreat Sci 26:219-226 (2019). Read more (PubMed: 30945470) »
  • Zhu Z  et al. Aucubin suppresses Titanium particles-mediated apoptosis of MC3T3-E1 cells and facilitates osteogenesis by affecting the BMP2/Smads/RunX2 signaling pathway. Mol Med Rep 18:2561-2570 (2018). Read more (PubMed: 30015916) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for contacting us.

We currently have two rabbit polyclonal antibodies that will detect SMAD 1/5:

Rabbit polyclonal to SMAD1/5/9 - ab80255
Rabbit polyclonal to SMAD 1/5/8/9 - ab13723

We have a number of antibodies that will detect the S463 or S465 phosphorylation on SMAD 1 or SMAD 5:

Rabbit polyclonal to Smad1 (phospho S465)- ab63439
Rabbit monoclonal [EP728(2)AY] to SMAD5 (phospho S463 + S465)- ab76296
Rabbit monoclonal [MMC-1-104-3] to SMAD5 (phospho S463 + S465) - ab92698
Rabbit polyclonal to Smad1 (phospho S465)- ab97689
Rabbit polyclonal to Smad1 (phospho S465)- ab97716

While we only currently carry antibodies raised in rabbit for these targets, you could use our EasyLink conjugation kits to conjugate these antibodies to Cy5.5 or Cy7. These two fluorophores should be compatible with the Odyssey system.


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Thank you for your enquiry. Following some searching of the relevant databases it seems that Smad8 is the previous name of Smad9. Please see the following references: http://marvester.embl.de/marvester/Q9JI/Q9JIW5.htm http://nsm1.utdallas.edu/bio/Dmello/DEV-NEURO/Neural%20induction/Review-3.pdf I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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