Recombinant
RabMAb

Recombinant Anti-Smad1 antibody [EP565Y] (ab33902)

Reviews (4) Submit a question References (15)

Overview

  • Product name

    Anti-Smad1 antibody [EP565Y]
    See all Smad1 primary antibodies

  • Description

    Rabbit monoclonal [EP565Y] to Smad1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human Smad1 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa cells. WB: HeLa whole cell lysate (ab150035).

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab33902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/500 - 1/2000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC or IP.

    • Target

    • Function

      Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.

    • Tissue specificity

      Ubiquitous. Highest expression seen in the heart and skeletal muscle.

    • Sequence similarities

      Belongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.

    • Post-translational
      modifications

      Phosphorylated on serine by BMP type 1 receptor kinase.
      Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.

    • Cellular localization

      Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • BSP-1 antibody
        • BSP1 antibody
        • HsMAD1 antibody
        • JV4-1 antibody
        • JV41 antibody
        • MAD homolog 1 antibody
        • MAD mothers against decapentaplegic homolog 1 antibody
        • Mad related protein 1 antibody
        • Mad-related protein 1 antibody
        • MADH1 antibody
        • MADR1 antibody
        • Mothers against decapentaplegic homolog 1 antibody
        • Mothers against DPP homolog 1 antibody
        • SMA- AND MAD-RELATED PROTEIN 1 antibody
        • SMAD 1 antibody
        • SMAD family member 1 antibody
        • SMAD mothers against DPP homolog 1 antibody
        • Smad1 antibody
        • SMAD1_HUMAN antibody
        • TGF beta signaling protein 1 antibody
        • Transforming growth factor-beta-signaling protein 1 antibody
        see all

      Images

      • Western blot - Anti-Smad1 antibody [EP565Y] (ab33902)
        Western blot - Anti-Smad1 antibody [EP565Y] (ab33902)
        Anti-Smad1 antibody [EP565Y] (ab33902) at 1/500 dilution + HeLa cell lysate

        Predicted band size: 52 kDa
        Observed band size: 58 kDa
        why is the actual band size different from the predicted?

      • Immunocytochemistry/ Immunofluorescence - Anti-Smad1 antibody [EP565Y] (ab33902)
        Immunocytochemistry/ Immunofluorescence - Anti-Smad1 antibody [EP565Y] (ab33902)
        Ab33902, at 1/100 dilution, staining Smad1 in HeLa cells by Immunocytochemistry/Immunofluorescence.

      References

      This product has been referenced in:

      • Diederichs S  et al. Regulation of WNT5A and WNT11 during MSC in vitro chondrogenesis: WNT inhibition lowers BMP and hedgehog activity, and reduces hypertrophy. Cell Mol Life Sci N/A:N/A (2019). Read more (PubMed: 30980110) »
      • Ruvolo PP  et al. Role of MSC-derived galectin 3 in the AML microenvironment. Biochim Biophys Acta 1865:959-969 (2018). Read more (PubMed: 29655803) »
      See all 15 Publications for this product

      Publishing research using ab33902? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-4 of 4 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Smad1 antibody [EP565Y]

       Good
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (primary monocytes)
      Permeabilization
      No
      Specification
      primary monocytes
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 09 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Smad1 antibody [EP565Y]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (skin)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate buffer pH6
      Permeabilization
      No
      Specification
      skin
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      10% buffered formalin
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 29 2015

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Smad1 antibody [EP565Y]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (pancreatic tumor)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
      Permeabilization
      No
      Specification
      pancreatic tumor
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      10% buffered formalin
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 29 2015

      Western blot abreview for Anti-Smad1 antibody [EP565Y]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - nuclear (Normal Human Keratinocyte)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      20 µg
      Specification
      Normal Human Keratinocyte
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 15 2015

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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