Recombinant Anti-Smad1 antibody [EP565Y] (ab33902)
- Datasheet
- References (17)
- Protocols
Overview
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Product name
Anti-Smad1 antibody [EP565Y]
See all Smad1 primary antibodies -
Description
Rabbit monoclonal [EP565Y] to Smad1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Smad1 aa 150-250. The exact sequence is proprietary.
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Positive control
- ICC/IF: HeLa cells. WB: HeLa whole cell lysate (ab150035).
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP565Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab33902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| WB | 1/500 - 1/2000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa). |
Target
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Function
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. -
Tissue specificity
Ubiquitous. Highest expression seen in the heart and skeletal muscle. -
Sequence similarities
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
Post-translational
modificationsPhosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4086 Human
- Entrez Gene: 17125 Mouse
- Omim: 601595 Human
- SwissProt: Q15797 Human
- SwissProt: P70340 Mouse
- Unigene: 604588 Human
- Unigene: 223717 Mouse
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Alternative names
- BSP-1 antibody
- BSP1 antibody
- HsMAD1 antibody
see all
Images
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![Western blot - Anti-Smad1 antibody [EP565Y] (ab33902)](https://www.abcam.com/ps/products/33/ab33902/Images/ab33902-47280-anti-smad1-antibody-ep565y-western-blot.jpg)
Western blot - Anti-Smad1 antibody [EP565Y] (ab33902)Anti-Smad1 antibody [EP565Y] (ab33902) at 1/500 dilution + HeLa cell lysate
Predicted band size: 52 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-Smad1 antibody [EP565Y] (ab33902)](https://www.abcam.com/ps/products/33/ab33902/Images/ab33902-47279-anti-smad1-antibody-ep565y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Smad1 antibody [EP565Y] (ab33902)Ab33902, at 1/100 dilution, staining Smad1 in HeLa cells by Immunocytochemistry/Immunofluorescence.
References
This product has been referenced in:
- Diederichs S et al. Regulation of WNT5A and WNT11 during MSC in vitro chondrogenesis: WNT inhibition lowers BMP and hedgehog activity, and reduces hypertrophy. Cell Mol Life Sci N/A:N/A (2019). Read more (PubMed: 30980110) »
- Esakky P et al. Testicular cells exhibit similar molecular responses to cigarette smoke condensate ex vivo and in vivo. FASEB J 32:63-72 (2018). Read more (PubMed: 28842431) »
