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Signal Transduction Signaling Pathway Nuclear Signaling SMADs
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RecombinantRabMAb

Recombinant Anti-Smad1 antibody [EP565Y] (ab33902)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (20)

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Western blot - Anti-Smad1 antibody [EP565Y] (ab33902)
  • Immunocytochemistry/ Immunofluorescence - Anti-Smad1 antibody [EP565Y] (ab33902)
  • Flow Cytometry - Anti-Smad1 antibody [EP565Y] (ab33902)
  • Anti-Smad1 antibody [EP565Y] (ab33902)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP565Y] to Smad1
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-Smad1 antibody [EP565Y]
    See all Smad1 primary antibodies
  • Description

    Rabbit monoclonal [EP565Y] to Smad1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Smad1 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: HEK-293, A-673, NIH/3T3 and C2C12 cell lysates; Mouse brain, skeletal muscle and stomach tissue lysates. Flow Cyt: HeLa Cells ICC/IF: HeLa cells
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP565Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • SMADs
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • SMADs
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Cytoplasmic
    • Cancer
    • Growth factors
    • TGF

Associated products

  • Alternative Versions

    • Anti-Smad1 antibody [EP565Y] - BSA and Azide free (ab172642)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human Smad1 protein (ab84653)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab33902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
WB
Mouse
Human
Application Abreviews Notes
Flow Cyt
1/100.
ICC/IF
1/100.
WB (1)
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
Notes
Flow Cyt
1/100.
ICC/IF
1/100.
WB
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).

Target

  • Function

    Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
  • Tissue specificity

    Ubiquitous. Highest expression seen in the heart and skeletal muscle.
  • Sequence similarities

    Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications

    Phosphorylated on serine by BMP type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
  • Target information above from: UniProt accession Q15797 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4086 Human
    • Entrez Gene: 17125 Mouse
    • Omim: 601595 Human
    • SwissProt: Q15797 Human
    • SwissProt: P70340 Mouse
    • Unigene: 604588 Human
    • Unigene: 223717 Mouse
    • Alternative names

      • BSP-1 antibody
      • BSP1 antibody
      • HsMAD1 antibody
      • JV4-1 antibody
      • JV41 antibody
      • MAD homolog 1 antibody
      • MAD mothers against decapentaplegic homolog 1 antibody
      • Mad related protein 1 antibody
      • Mad-related protein 1 antibody
      • MADH1 antibody
      • MADR1 antibody
      • Mothers against decapentaplegic homolog 1 antibody
      • Mothers against DPP homolog 1 antibody
      • SMA- AND MAD-RELATED PROTEIN 1 antibody
      • SMAD 1 antibody
      • SMAD family member 1 antibody
      • SMAD mothers against DPP homolog 1 antibody
      • Smad1 antibody
      • SMAD1_HUMAN antibody
      • TGF beta signaling protein 1 antibody
      • Transforming growth factor-beta-signaling protein 1 antibody
      see all

    Images

    • Western blot - Anti-Smad1 antibody [EP565Y] (ab33902)
      Western blot - Anti-Smad1 antibody [EP565Y] (ab33902)
      All lanes : Anti-Smad1 antibody [EP565Y] (ab33902) at 1/1000 dilution (Purified)

      Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
      Lane 2 : A-673 (Human muscle Ewing's Sarcoma) whole cell lysates
      Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
      Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
      Lane 5 : Mouse brain lysates
      Lane 6 : Mouse skeletal muscle lysates
      Lane 7 : Mouse stomach lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 52 kDa
      Observed band size: 58 kDa why is the actual band size different from the predicted?



      Blocking/Diluting buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-Smad1 antibody [EP565Y] (ab33902)
      Immunocytochemistry/ Immunofluorescence - Anti-Smad1 antibody [EP565Y] (ab33902)
      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Smad1 with Purified ab33902 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Flow Cytometry - Anti-Smad1 antibody [EP565Y] (ab33902)
      Flow Cytometry - Anti-Smad1 antibody [EP565Y] (ab33902)
      Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Smad1 with Purified ab33902 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • Anti-Smad1 antibody [EP565Y] (ab33902)
      Anti-Smad1 antibody [EP565Y] (ab33902)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (20)

    Publishing research using ab33902? Please let us know so that we can cite the reference in this datasheet.

    ab33902 has been referenced in 20 publications.

    • Diederichs S  et al. Regulation of WNT5A and WNT11 during MSC in vitro chondrogenesis: WNT inhibition lowers BMP and hedgehog activity, and reduces hypertrophy. Cell Mol Life Sci N/A:N/A (2019). PubMed: 30980110
    • Papoutsoglou P  et al. The TGFB2-AS1 lncRNA Regulates TGF-ß Signaling by Modulating Corepressor Activity. Cell Rep 28:3182-3198.e11 (2019). PubMed: 31533040
    • Zhang Y  et al. MicroRNA-23a inhibits osteogenesis of periodontal mesenchymal stem cells by targeting bone morphogenetic protein signaling. Arch Oral Biol 102:93-100 (2019). PubMed: 30981077
    • Alsina-Sanchís E  et al. ALK1 Loss Results in Vascular Hyperplasia in Mice and Humans Through PI3K Activation. Arterioscler Thromb Vasc Biol 38:1216-1229 (2018). PubMed: 29449337
    • Esakky P  et al. Testicular cells exhibit similar molecular responses to cigarette smoke condensate ex vivo and in vivo. FASEB J 32:63-72 (2018). PubMed: 28842431
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Smad1 antibody [EP565Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate buffer pH6
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 29 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Smad1 antibody [EP565Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (pancreatic tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
    Permeabilization
    No
    Specification
    pancreatic tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 29 2015

    Western blot abreview for Anti-Smad1 antibody [EP565Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Normal Human Keratinocyte)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Normal Human Keratinocyte
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 15 2015

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