Product nameAnti-Smad1 antibody [EP565Y]
See all Smad1 primary antibodies
DescriptionRabbit monoclonal [EP565Y] to Smad1
Tested applicationsSuitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Smad1 aa 150-250. The exact sequence is proprietary.
- ICC/IF: HeLa cells. WB: HeLa whole cell lysate (ab150035).
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab33902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/500 - 1/2000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).|
FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
Sequence similaritiesBelongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
modificationsPhosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
- Information by UniProt
- BSP-1 antibody
- BSP1 antibody
- HsMAD1 antibody
Anti-Smad1 antibody [EP565Y] (ab33902) at 1/500 dilution + HeLa cell lysate
Predicted band size: 52 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Ab33902, at 1/100 dilution, staining Smad1 in HeLa cells by Immunocytochemistry/Immunofluorescence.
This product has been referenced in:
- Diederichs S et al. Regulation of WNT5A and WNT11 during MSC in vitro chondrogenesis: WNT inhibition lowers BMP and hedgehog activity, and reduces hypertrophy. Cell Mol Life Sci N/A:N/A (2019). Read more (PubMed: 30980110) »
- Esakky P et al. Testicular cells exhibit similar molecular responses to cigarette smoke condensate ex vivo and in vivo. FASEB J 32:63-72 (2018). Read more (PubMed: 28842431) »