Recombinant Anti-Smad1 antibody [EP565Y] (ab33902)
![Western blot - Anti-Smad1 antibody [EP565Y] (ab33902)](https://www.abcam.com/ps/products/33/ab33902/Images/ab33902-392505-anti-smad1-antibody-ep565y-westernblot-human-mouse.jpg "Western blot - Anti-Smad1 antibody [EP565Y] (ab33902)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP565Y] to Smad1
- Suitable for: Flow Cyt, ICC/IF, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Smad1 antibody [EP565Y]
See all Smad1 primary antibodies -
Description
Rabbit monoclonal [EP565Y] to Smad1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human Smad1 aa 150-250. The exact sequence is proprietary.
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Positive control
- WB: HEK-293, A-673, NIH/3T3 and C2C12 cell lysates; Mouse brain, skeletal muscle and stomach tissue lysates. Flow Cyt: HeLa Cells ICC/IF: HeLa cells
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP565Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
|
| WB |
Mouse
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/100.
|
|
| ICC/IF |
1/100.
|
|
| WB | (1) |
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
|
| Notes |
|---|
|
Flow Cyt
1/100. |
|
ICC/IF
1/100. |
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WB
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa). |
Target
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Function
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. -
Tissue specificity
Ubiquitous. Highest expression seen in the heart and skeletal muscle. -
Sequence similarities
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
Post-translational
modificationsPhosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4086 Human
- Entrez Gene: 17125 Mouse
- Omim: 601595 Human
- SwissProt: Q15797 Human
- SwissProt: P70340 Mouse
- Unigene: 604588 Human
- Unigene: 223717 Mouse
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Alternative names
- BSP-1 antibody
- BSP1 antibody
- HsMAD1 antibody
see all
Images
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Western blot - Anti-Smad1 antibody [EP565Y] (ab33902)All lanes : Anti-Smad1 antibody [EP565Y] (ab33902) at 1/1000 dilution (Purified)
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : A-673 (Human muscle Ewing's Sarcoma) whole cell lysates
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 5 : Mouse brain lysates
Lane 6 : Mouse skeletal muscle lysates
Lane 7 : Mouse stomach lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-Smad1 antibody [EP565Y] (ab33902)](https://www.abcam.com/ps/products/33/ab33902/Images/ab33902-6-anti-smad1-antibody-ep565y-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Smad1 antibody [EP565Y] (ab33902)Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Smad1 with Purified ab33902 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Flow Cytometry - Anti-Smad1 antibody [EP565Y] (ab33902)](https://www.abcam.com/ps/products/33/ab33902/Images/ab33902-7-anti-smad1-antibody-ep565y-flowcytometry-hela-human.jpg)
Flow Cytometry - Anti-Smad1 antibody [EP565Y] (ab33902)Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Smad1 with Purified ab33902 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). -
![Anti-Smad1 antibody [EP565Y] (ab33902)](https://www.abcam.com/ps/products/33/ab33902/Images/ab33902-8-benefits-of-recombinant-antibodies.png)
Anti-Smad1 antibody [EP565Y] (ab33902)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab33902 has been referenced in 20 publications.
- Diederichs S et al. Regulation of WNT5A and WNT11 during MSC in vitro chondrogenesis: WNT inhibition lowers BMP and hedgehog activity, and reduces hypertrophy. Cell Mol Life Sci N/A:N/A (2019). PubMed: 30980110
- Papoutsoglou P et al. The TGFB2-AS1 lncRNA Regulates TGF-ß Signaling by Modulating Corepressor Activity. Cell Rep 28:3182-3198.e11 (2019). PubMed: 31533040
- Zhang Y et al. MicroRNA-23a inhibits osteogenesis of periodontal mesenchymal stem cells by targeting bone morphogenetic protein signaling. Arch Oral Biol 102:93-100 (2019). PubMed: 30981077
- Alsina-Sanchís E et al. ALK1 Loss Results in Vascular Hyperplasia in Mice and Humans Through PI3K Activation. Arterioscler Thromb Vasc Biol 38:1216-1229 (2018). PubMed: 29449337
- Esakky P et al. Testicular cells exhibit similar molecular responses to cigarette smoke condensate ex vivo and in vivo. FASEB J 32:63-72 (2018). PubMed: 28842431