Recombinant
RabMAb

Anti-Smad1 antibody [EPR5522] (ab126761)

Overview

  • Product name
    Anti-Smad1 antibody [EPR5522]
    See all Smad1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5522] to Smad1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Smad1 aa 200-300. The exact sequence is proprietary.

  • Positive control
    • HeLa, A673, HepG2, and 293T cell lysate; Human colon tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
    • Tissue specificity
      Ubiquitous. Highest expression seen in the heart and skeletal muscle.
    • Sequence similarities
      Belongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.
    • Post-translational
      modifications
      Phosphorylated on serine by BMP type 1 receptor kinase.
      Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
    • Cellular localization
      Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • BSP-1 antibody
      • BSP1 antibody
      • HsMAD1 antibody
      • JV4-1 antibody
      • JV41 antibody
      • MAD homolog 1 antibody
      • MAD mothers against decapentaplegic homolog 1 antibody
      • Mad related protein 1 antibody
      • Mad-related protein 1 antibody
      • MADH1 antibody
      • MADR1 antibody
      • Mothers against decapentaplegic homolog 1 antibody
      • Mothers against DPP homolog 1 antibody
      • SMA- AND MAD-RELATED PROTEIN 1 antibody
      • SMAD 1 antibody
      • SMAD family member 1 antibody
      • SMAD mothers against DPP homolog 1 antibody
      • Smad1 antibody
      • SMAD1_HUMAN antibody
      • TGF beta signaling protein 1 antibody
      • Transforming growth factor-beta-signaling protein 1 antibody
      see all

    Images

    • All lanes : Anti-Smad1 antibody [EPR5522] (ab126761) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A673 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size: 52 kDa

    • ab126761, at 1/50 dilution, staining Smad1 in paraffin-embedded Human colon tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126761 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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