Recombinant Anti-Smad1 antibody [EPR5522] - BSA and Azide free (ab176885)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Smad1 antibody [EPR5522] - BSA and Azide free
See all Smad1 primary antibodies -
Description
Rabbit monoclonal [EPR5522] to Smad1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Smad1 aa 200-300. The exact sequence is proprietary.
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General notes
Ab176885 is the carrier-free version of ab126761. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab176885 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 2.56 x 10 -11 M Learn more about KD -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5522 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab176885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa). |
Target
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Function
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. -
Tissue specificity
Ubiquitous. Highest expression seen in the heart and skeletal muscle. -
Sequence similarities
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
Post-translational
modificationsPhosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4086 Human
- Omim: 601595 Human
- SwissProt: Q15797 Human
- Unigene: 604588 Human
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Alternative names
- BSP-1 antibody
- BSP1 antibody
- HsMAD1 antibody
see all
Datasheets and documents
References
ab176885 has not yet been referenced specifically in any publications.
