Product nameAnti-Smad1 (phospho S206) antibody
See all Smad1 primary antibodies
DescriptionRabbit polyclonal to Smad1 (phospho S206)
Specificityab106093 is specific for Human Smad1 phosphorylated at pS206. Reactivity with nonphosphorylated Human Smad1 is minimal by ELISA and western blot.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Drosophila melanogaster, Macaque monkey, Gorilla, Opossum, Orangutan
A phosphorylated synthetic peptide corresponding to amino acids surrounding serine 206 of Human Smad1.
- HEPG2 whole cell lysates treated with BMP2, EGF, or NaCl.
General notesExpiration date is one year from date of opening.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab1060093 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.
Our Abpromise guarantee covers the use of ab106093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 52 kDa.|
|ELISA||1/5000 - 1/25000.|
FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
Sequence similaritiesBelongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
modificationsPhosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
- Information by UniProt
- BSP-1 antibody
- BSP1 antibody
- HsMAD1 antibody
All lanes : Anti-Smad1 (phospho S206) antibody (ab106093) at 1/500 dilution
Lane 1 : HepG2 whole cell lysate, treated with PBS
Lanes 2-3 : HepG2 whole cell lysate, treated with BMP2 (5 ng/ml)
Lane 4 : HepG2 whole cell lysate, treated with NaCL
Lysates/proteins at 15 µg per lane.
All lanes : HRP-conjugated a-Rabbit IgG at 1/3000 dilution
Predicted band size: 52 kDa
Cell lysates were incubated for 1 h at 37°C.
Primary antibody was pre-incubated before reacting with blot as follows:
top row - with PBS,
middle row - with the immunizing phosphorylated peptide
bottom row - with control or non-phosphorylated peptide.
ab106093 has not yet been referenced specifically in any publications.