Overview

  • Product name

    Anti-Smad1 (phospho S465) antibody
    See all Smad1 primary antibodies
  • Description

    Rabbit polyclonal to Smad1 (phospho S465)
  • Host species

    Rabbit
  • Specificity

    ab97689 detects endogenous levels of Smad1 only when phosphorylated at serine 465. The region surrounding Smad1 phospho S465 is highly conserved in several other Smad proteins, including Smad 5 and Smad 9. Cross reactivity with other Smad proteins is likely.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from Human Smad1 around the phosphorylation site of serine 465 (I-S-S-V-SP).

  • Positive control

    • HeLa cell extracts treated with PMA.

Properties

Applications

Our Abpromise guarantee covers the use of ab97689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).

Target

  • Function

    Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
  • Tissue specificity

    Ubiquitous. Highest expression seen in the heart and skeletal muscle.
  • Sequence similarities

    Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications

    Phosphorylated on serine by BMP type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BSP-1 antibody
    • BSP1 antibody
    • HsMAD1 antibody
    • JV4-1 antibody
    • JV41 antibody
    • MAD homolog 1 antibody
    • MAD mothers against decapentaplegic homolog 1 antibody
    • Mad related protein 1 antibody
    • Mad-related protein 1 antibody
    • MADH1 antibody
    • MADR1 antibody
    • Mothers against decapentaplegic homolog 1 antibody
    • Mothers against DPP homolog 1 antibody
    • SMA- AND MAD-RELATED PROTEIN 1 antibody
    • SMAD 1 antibody
    • SMAD family member 1 antibody
    • SMAD mothers against DPP homolog 1 antibody
    • Smad1 antibody
    • SMAD1_HUMAN antibody
    • TGF beta signaling protein 1 antibody
    • Transforming growth factor-beta-signaling protein 1 antibody
    see all

Images

  • All lanes : Anti-Smad1 (phospho S465) antibody (ab97689) at 1/500 dilution

    Lane 1 : HeLa cell extracts, untreated
    Lane 2 : HeLa cell extracts, treated with PMA

    Predicted band size: 52 kDa

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Xenopus laevis Tissue sections (st.10.5 embryo)
Permeabilization
No
Specification
st.10.5 embryo
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 20 2016

Answer

Thank you for contacting us.

We cannot disclose our exact immunogen sequence of ab63439. However, the amino acid range of this immunogen is aa415-465 of human Mothers against decapentaplegic homolog 1 with ACC#Q15797 (http://www.uniprot.org/uniprot/Q15797).

I was unable to obtain further information regarding ab97689 but will pass along anything further I receive.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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