Product nameAnti-SMAD1/5/9 antibody
See all SMAD1/5/9 primary antibodies
DescriptionRabbit polyclonal to SMAD1/5/9
Specificityab80255 detects endogenous levels of total SMAD1/5/9 protein.
Tested applicationsSuitable for: IHC-FoFr, WB, ELISAmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Synthetic peptide derived from N terminal of human SMAD1/5/9
- HeLa cell extracts (WB), P7 rat cortex (IHC)
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab80255 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab80255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).|
RelevanceSMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs, common-mediator SMAD and inhibitory SMADs. SMAD1, SMAD5 and SMAD9 have high degrees of homology. SMAD8 and SMAD9 are used as alternate names for the same protein. Human SMAD1 is a 465 amino acid protein, human SMAD5 is a 465 amino acid and human SMAD9 is a 430 amino acid protein.
Cellular localizationCytoplasmic and Nuclear
- AI528653 antibody
- BSP1 antibody
- DKFZp781C1895 antibody
All lanes : Anti-SMAD1/5/9 antibody (ab80255) at 1/500 dilution
Lane 1 : HeLa cell extracts
Lane 2 : HeLa cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Immunohistomchemical detection of SMAD1/5/9 using antibody ab80255 on perfusion fixed P7 rat cortex sections. The animals were perfused with 4% PFA. The sections were incubated in 10% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab80255, 1µg/ml) and (ab15640) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 568 (red) at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Lui JC et al. Differential aging of growth plate cartilage underlies differences in bone length and thus helps determine skeletal proportions. PLoS Biol 16:e2005263 (2018). Read more (PubMed: 30036371) »
- Loscertales M et al. Type IV collagen drives alveolar epithelial-endothelial association and the morphogenetic movements of septation. BMC Biol 14:59 (2016). IHC . Read more (PubMed: 27412481) »