Product nameAnti-Smad2 + Smad3 antibody
See all Smad2 + Smad3 primary antibodies
DescriptionRabbit polyclonal to Smad2 + Smad3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human
Synthetic peptide within Human Smad2 + Smad3 aa 30-80 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q15796
- Mouse lymphoma tissue; mouse brain lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Aqueous buffered solution
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab217553 in the following tested applications.
|WB||1/100 - 1/1000.|
|IHC-P||1/100 - 1/500.|
RelevanceSMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
Cellular localizationCytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
- hMAD 2 antibody
- hMAD 3 antibody
- hSMAD2 antibody
All lanes : Anti-Smad2 + Smad3 antibody (ab217553) at 1/200 dilution
Lane 1 : mouse brain lysates
Lane 2 : mouse kidney lysates
All lanes : HRP-conjugated secondary antibody at 1/3000 dilution
Observed band size: 47 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of formalin-fixed and paraffin-embedded mouse lymphoma tissue labeling Smad2 + Smad3 with ab217553 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
This product has been referenced in:
- Li L et al. The dipeptidyl peptidase-4 inhibitor sitagliptin ameliorates renal injury in type 1 diabetic mice via inhibiting the TGF-ß/Smad signal pathway. Pharmazie 74:239-242 (2019). Read more (PubMed: 30940309) »
- Huang T et al. STK39, overexpressed in osteosarcoma, regulates osteosarcoma cell invasion and proliferation. Oncol Lett 14:4599-4604 (2017). WB ; Human . Read more (PubMed: 28943960) »