Anti-Smad2 + Smad3 antibody (ab217553)

Rabbit polyclonal Smad2 + Smad3 antibody. Validated in WB, IHC and tested in Mouse. Cited in 1 publication(s). Immunogen corresponding to synthetic peptide.


  • Product name

    Anti-Smad2 + Smad3 antibody
    See all Smad2 + Smad3 primary antibodies
  • Description

    Rabbit polyclonal to Smad2 + Smad3
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Smad2 + Smad3 aa 30-80 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q15796

  • Positive control

    • Mouse lymphoma tissue; mouse brain lysates.



Our Abpromise guarantee covers the use of ab217553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000.
IHC-P 1/100 - 1/500.


  • Relevance

    SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
  • Cellular localization

    Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
  • Database links

  • Alternative names

    • hMAD 2 antibody
    • hMAD 3 antibody
    • hSMAD2 antibody
    • hSMAD3 antibody
    • Mad related protein 2 antibody
    • MADH2 antibody
    • MADH3 antibody
    • MADR2 antibody
    • Mothers against DPP homolog 2 antibody
    • Mothers against DPP homolog 3 antibody
    • Sma and Mad related protein 2 antibody
    • SMA and MAD related protein 3 antibody
    • SMAD 2 antibody
    • SMAD 3 antibody
    • SMAD family member 2 antibody
    • SMAD family member 3 antibody
    • smad2+3 antibody
    • smad2/3 antibody
    see all


  • All lanes : Anti-Smad2 + Smad3 antibody (ab217553) at 1/200 dilution

    Lane 1 : mouse brain lysates
    Lane 2 : mouse kidney lysates

    All lanes : HRP-conjugated secondary antibody at 1/3000 dilution

    Observed band size: 47 kDa
    why is the actual band size different from the predicted?

    12% SDS-PAGE

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded mouse lymphoma tissue labeling Smad2 + Smad3 with ab217553 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.


This product has been referenced in:

  • Li L  et al. The dipeptidyl peptidase-4 inhibitor sitagliptin ameliorates renal injury in type 1 diabetic mice via inhibiting the TGF-ß/Smad signal pathway. Pharmazie 74:239-242 (2019). Read more (PubMed: 30940309) »
  • Huang T  et al. STK39, overexpressed in osteosarcoma, regulates osteosarcoma cell invasion and proliferation. Oncol Lett 14:4599-4604 (2017). WB ; Human . Read more (PubMed: 28943960) »
See all 2 Publications for this product

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