Anti-Smad2 antibody [5G7] (ab119907)
Key features and details
- Mouse monoclonal [5G7] to Smad2
- Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Smad2 antibody [5G7]
See all Smad2 primary antibodies -
Description
Mouse monoclonal [5G7] to Smad2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Purified recombinant fragment of Human Smad2 expressed in E. Coli.
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Positive control
- Recombinant protein; Smad2-hIgGFc transfected HEK293 cell lysate; Human liver cancer and cerebellum tissues; U251 and NIH3T3 cells.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR163661-5 are from Tissue Culture Supernatant
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5G7 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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ELISA |
1/10000.
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IHC-P |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/200 - 1/1000.
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Notes |
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WB
1/500 - 1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
ELISA
1/10000. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/200 - 1/1000. |
Target
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Function
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. -
Tissue specificity
Expressed at high levels in skeletal muscle, heart and placenta. -
Sequence similarities
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
Post-translational
modificationsPhosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. - Information by UniProt
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Database links
- Entrez Gene: 4087 Human
- Entrez Gene: 17126 Mouse
- Omim: 601366 Human
- SwissProt: Q15796 Human
- SwissProt: Q62432 Mouse
- Unigene: 12253 Human
- Unigene: 705764 Human
- Unigene: 391091 Mouse
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Alternative names
- Drosophila, homolog of, MADR2 antibody
- hMAD-2 antibody
- HsMAD2 antibody
see all
Images
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Anti-Smad2 antibody [5G7] (ab119907) at 1/500 dilution + Recombinant Human Smad2 protein
Predicted band size: 52 kDa -
All lanes : Anti-Smad2 antibody [5G7] (ab119907) at 1/500 dilution
Lane 1 : Non-transfected HEK293 cell lysate.
Lane 2 : Smad2-hIgGFc transfected HEK293 cell lysate.
Predicted band size: 52 kDa -
ab119907 at 1/200 dilution staining Smad2 in paraffin-embedded Human liver cancer tissues by Immunohistochemistry followed by DAB staining.
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ab119907 at 1/200 dilution staining Smad2 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry followed by DAB staining.
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ab119907 at 1/200 dilution staining Smad2 in U251 cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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ELISA showing use of ab119907.
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ab119907 at 1/200 dilution staining Smad2 in NIH3T3 cells by Flow Cytometry (blue) and negative control (red).
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab119907 has been referenced in 6 publications.
- Wang J et al. High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells. Front Oncol 11:716246 (2021). PubMed: 34745942
- Yoon S et al. TGF-ß-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance. Cell Rep 31:107685 (2020). PubMed: 32460012
- Zeng K et al. PAX3 silencing inhibits prostate cancer progression through the suppression of the TGF-β/Smad signaling axis. Cell Biol Int 44:2131-2139 (2020). PubMed: 32672875
- Loeffler I et al. FSP1-specific SMAD2 knockout in renal tubular, endothelial, and interstitial cells reduces fibrosis and epithelial-to-mesenchymal transition in murine STZ-induced diabetic nephropathy. Cell Tissue Res 372:115-133 (2018). PubMed: 29209813
- Fang L et al. Jumonji AT-rich interactive domain 1B overexpression is associated with the development and progression of glioma. Int J Mol Med 38:172-82 (2016). WB ; Human . PubMed: 27246838
- Zheng J et al. Activation of hepatic stellate cells is suppressed by microRNA-150. Int J Mol Med 32:17-24 (2013). PubMed: 23604143