Product nameAnti-Smad2 antibody [7A5]
See all Smad2 primary antibodies
DescriptionMouse monoclonal [7A5] to Smad2
Tested applicationsSuitable for: ChIP, WB, IP, ELISA, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length human Smad2 protein purified from E.coli with His tag.
- HeLa, A431, NIH3T3 and PC12 cell lysates.
This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR183929 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: HEPES, 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride
PurityTissue culture supernatant
Light chain typekappa
Our Abpromise guarantee covers the use of ab71109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).|
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionReceptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.
Tissue specificityExpressed at high levels in skeletal muscle, heart and placenta.
Sequence similaritiesBelongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
modificationsPhosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.
- Information by UniProt
- Drosophila, homolog of, MADR2 antibody
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Overlay histogram showing PC3 cells stained with ab71109 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab71109, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
All lanes : Anti-Smad2 antibody [7A5] (ab71109) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : NIH3T3 cell lysate
Lane 4 : PC12 cell lysate
Predicted band size: 58 kDa
Observed band size: 58 kDa
This product has been referenced in:
- Zhang H et al. Excessive retinoic acid inhibit mouse embryonic palate mesenchymal cell growth through involvement of Smad signaling. Anim Cells Syst (Seoul) 21:31-36 (2017). Read more (PubMed: 30460049) »
- Pereira PN et al. Antagonism of Nodal signaling by BMP/Smad5 prevents ectopic primitive streak formation in the mouse amnion. Development 139:3343-54 (2012). Read more (PubMed: 22912414) »