Application
Western blot
Sample
Human Cell lysate - whole cell (Human cell line HGL-5)
Loading amount
15 µg
Specification
Human cell line HGL-5
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/3000
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Detection
Detection method
Millipore WBK0500
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 58 kDa
Positive control
Jurkat Cells
Additional data
Additional Notes
Marker (Biorad Dual Color)
MII/MII (cells, no additives)
AII/MII (cells, plus activin A )
MII/CII ( cells,plus Cyclophilin siRNA Control)
AII/CII ( plus activin A, plus Cyclophilin)
MII/NII ( non targeting, negativ control for siRNA)
AII/NII (cells, plus activin A, negativ control for siRNA)
MII/SII ( cells, plus siRNA Smartpool)
AII/SII ( cells, plus Activin A, plus siRNA Smartpool)
Jurkat Cells
in total nine samples were analysed
MII/MII (cells, no additives)
AII/MII (cells, plus activin A )
MII/CII ( cells,plus Cyclophilin siRNA Control)
AII/CII ( plus activin A, plus Cyclophilin)
MII/NII ( non targeting, negativ control for siRNA)
AII/NII (cells, plus activin A, negativ control for siRNA)
MII/SII ( cells, plus siRNA Smartpool)
AII/SII ( cells, plus Activin A, plus siRNA Smartpool)
Jurkat Cells
in total nine samples were analysed
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Birte Münchow
Verified customer
Submitted Oct 17 2006