Application
Western blot
Sample
Mouse Cell lysate - whole cell (c3h10t1/2)
Loading amount
25 µg
Specification
c3h10t1/2
Treatment
TGFbeta 15 and 30 minutes
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5%BSA TBS/T
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 51 kDa
Additional data
Additional Notes
1 - Untreated 2 - TGFbeta1 - 15 min. 3 - TGFbeta1 - 30 min.
We do not plan to run these samples alongside human samples because we do not have them. We believe, however, that the observed bands are smad2 because we observed identical bands using another company's smad2 antibody.
We do not plan to run these samples alongside human samples because we do not have them. We believe, however, that the observed bands are smad2 because we observed identical bands using another company's smad2 antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Serban Georgescu
Verified customer
Submitted Mar 03 2008