Key features and details
- Rabbit polyclonal to Smad2 (phospho S467)
- Suitable for: Dot blot, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Smad2 (phospho S467) antibody
See all Smad2 primary antibodies
DescriptionRabbit polyclonal to Smad2 (phospho S467)
Tested applicationsSuitable for: Dot blot, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide. This information is considered to be commercially sensitive.
- IHC-P: Human brain cortex. ICC/IF: HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.|
|IHC-P||1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 15 µg/ml.|
FunctionReceptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.
Tissue specificityExpressed at high levels in skeletal muscle, heart and placenta.
Sequence similaritiesBelongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
modificationsPhosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.
- Information by UniProt
- Drosophila, homolog of, MADR2 antibody
- hMAD-2 antibody
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Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded human brain cortex tissue stained for Smad2 (phospho S467) using ab235265 at a 1/1000 dilution for 30 mins at RT, followed by a ready to use anti-rabbit poly HRP IgG for 8 mins at RT. Counterstained with hematoxylin.
HIER using citrate buffer for 20 mins was performed.
Dot Blot - Anti-Smad2 (phospho S467) antibody (ab235265 at 1 µg/ml).
Lanes 1-5: 100, 33.33, 11.11, 3.70, 1.23 ng.
Row A: Smad2 phospho-S467 - BSA Peptide.
Row B: Smad2 S467 - BSA Peptide.
Row C: AKT1 pS473 - BSA Peptide.
Row D: AKT1 S473 - BSA Peptide.
Row E: Scramble Sprouty - BSA Peptide.
Row F: BSA.
Exposure time of 10 secs.
Immunocytochemistry/ Immunofluorescence analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Smad2 (phospho S467) using ab235265 at 15 µg/ml overnight at 4°C. A donkey anti-rabbit IgG DyLight™ 488 was used as the secondary, 5 µg/ml for 1 hour at RT.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235265 has not yet been referenced specifically in any publications.