Overview

  • Product name

    Anti-Smad2 (phospho S467) antibody
    See all Smad2 primary antibodies
  • Description

    Rabbit polyclonal to Smad2 (phospho S467)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: Human brain cortex. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab235265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
IHC-P 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 15 µg/ml.

Target

  • Function

    Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.
  • Tissue specificity

    Expressed at high levels in skeletal muscle, heart and placenta.
  • Sequence similarities

    Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications

    Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
    In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
    Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.
  • Information by UniProt
  • Database links

  • Alternative names

    • Drosophila, homolog of, MADR2 antibody
    • hMAD-2 antibody
    • HsMAD2 antibody
    • JV18 antibody
    • JV18-1 antibody
    • JV181 antibody
    • MAD antibody
    • MAD homolog 2 antibody
    • MAD Related Protein 2 antibody
    • Mad-related protein 2 antibody
    • MADH2 antibody
    • MADR2 antibody
    • MGC22139 antibody
    • MGC34440 antibody
    • Mother against DPP homolog 2 antibody
    • Mothers against decapentaplegic homolog 2 antibody
    • Mothers against decapentaplegic, Drosophila, homolog of, 2 antibody
    • Mothers against DPP homolog 2 antibody
    • OTTHUMP00000163489 antibody
    • Sma and Mad related protein 2 antibody
    • Sma- and Mad-related protein 2 MAD antibody
    • SMAD 2 antibody
    • SMAD family member 2 antibody
    • SMAD, mothers against DPP homolog 2 antibody
    • SMAD2 antibody
    • SMAD2_HUMAN antibody
    see all

Images

  • Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded human brain cortex tissue stained for Smad2 (phospho S467) using ab235265 at a 1/1000 dilution for 30 mins at RT, followed by a ready to use anti-rabbit poly HRP IgG for 8 mins at RT. Counterstained with hematoxylin.

    HIER using citrate buffer for 20 mins was performed.

  • Dot Blot - Anti-Smad2 (phospho S467) antibody (ab235265 at 1 µg/ml).

    Lanes 1-5: 100, 33.33, 11.11, 3.70, 1.23 ng.

    Row A: Smad2 phospho-S467 - BSA Peptide.

    Row B: Smad2 S467 - BSA Peptide.

    Row C: AKT1 pS473 - BSA Peptide.

    Row D: AKT1 S473  - BSA Peptide.

    Row E: Scramble Sprouty - BSA Peptide.

    Row F: BSA.

    Exposure time of 10 secs.

     

  • Immunocytochemistry/ Immunofluorescence analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Smad2 (phospho S467) using ab235265 at 15 µg/ml overnight at 4°C. A donkey anti-rabbit IgG DyLight™ 488 was used as the secondary, 5 µg/ml for 1 hour at RT.

References

ab235265 has not yet been referenced specifically in any publications.

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