Overview

  • Product name

    Anti-Smad2 + Smad3 antibody
    See all Smad2 + Smad3 primary antibodies
  • Description

    Rabbit polyclonal to Smad2 + Smad3
  • Host species

    Rabbit
  • Specificity

    ab63672 detects endogenous levels of total Smad2 and Smad3.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic non-phosphopeptide derived from human Smad2 and Smad3 around the phosphorylation site of threonine 8 (P-F-TP-P-P).

  • Positive control

    • COS7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab63672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa.

Target

  • Relevance

    SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
  • Cellular localization

    Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
  • Database links

  • Alternative names

    • hMAD 2 antibody
    • hMAD 3 antibody
    • hSMAD2 antibody
    • hSMAD3 antibody
    • Mad related protein 2 antibody
    • MADH2 antibody
    • MADH3 antibody
    • MADR2 antibody
    • Mothers against DPP homolog 2 antibody
    • Mothers against DPP homolog 3 antibody
    • Sma and Mad related protein 2 antibody
    • SMA and MAD related protein 3 antibody
    • SMAD 2 antibody
    • SMAD 3 antibody
    • SMAD family member 2 antibody
    • SMAD family member 3 antibody
    • smad2+3 antibody
    • smad2/3 antibody
    see all

Images

  • All lanes : Anti-Smad2 + Smad3 antibody (ab63672) at 1/500 dilution

    Lane 1 : COS7 cell extracts treated with UV (15mins)
    Lane 2 : COS7 cell extracts untreated
    Lane 3 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins)
    Lane 4 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Observed band size: 48 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Ji J & He L Effect of Kangxianling Decoction on Expression of TGF-ß1/Smads and Extracellular Matrix Deposition. Evid Based Complement Alternat Med 2019:5813549 (2019). Read more (PubMed: 30713574) »
  • He WM  et al. Oleanolic acid attenuates TGF-ß1-induced epithelial-mesenchymal transition in NRK-52E cells. BMC Complement Altern Med 18:205 (2018). Read more (PubMed: 29973206) »
See all 8 Publications for this product

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