Western blot abreview for Anti-Smad3 antibody - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA 231 cell line)
Loading amount
25 µg
Specification
MDA 231 cell line
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBS-T

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit secondary HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: 48 kDa

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Submitted May 21 2009

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