Western blot abreview for Anti-Smad3 antibody - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Renal Proximal Tubular cells)
Loading amount
10 µg
Specification
Human Renal Proximal Tubular cells
Gel Running Conditions
Reduced Denaturing (7.5 %)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1 % BSA in PBS

Secondary antibody

Name
Non-Abcam antibody was used: DakoCytomation
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 50 kDa

Dr. Robert Jenkins

Verified customer

Submitted May 21 2009

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