Western blot abreview for Anti-Smad3 antibody - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3)
Loading amount
25 µg
Specification
NIH 3T3
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% Marvel TBS-T

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: 55 kDa

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Submitted May 20 2009

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