Immunocytochemistry/ Immunofluorescence abreview for Anti-Smad3 antibody - ChIP Grade

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (A549, Human TII Pneumocyte cell line)
Specification
A549, Human TII Pneumocyte cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

Other product details

Dilution
1/200
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in 1x PBST

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: TRITC
Dilution
1/200

Dr. Aaron Gardner

Verified customer

Submitted Nov 13 2009

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