Immunoprecipitation abreview for Anti-Smad3 antibody - ChIP Grade

Excellent
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (A549)
Total protein in input
500 µg
Specification
A549
Immuno-precipitation step
Protein G

Other product details

Concentration
10 µg/mg lysate
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS 0.5% NP40

Western Blot antibody

Dilution
1/2000

Additional data

Additional Notes
IP lysate was primarily used as part of an indirect ELISA for Smad3 (phospho S423 + S425) antibody [EP823Y] (ab52903). However a small sample was also tested via western blot, and the resulting band was present at the correct kda and displayed the same effect in result to treatment.

A Coomassie stain of lysate and wash was also used to assess efficacy.

Dr. Aaron Gardner

Verified customer

Submitted Dec 06 2010

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