IP lysate was primarily used as part of an indirect ELISA for Smad3 (phospho S423 + S425) antibody [EP823Y] (ab52903). However a small sample was also tested via western blot, and the resulting band was present at the correct kda and displayed the same effect in result to treatment.
A Coomassie stain of lysate and wash was also used to assess efficacy.
Dr. Aaron Gardner
Submitted Dec 06 2010
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