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Hello, I'm looking for antibodies that would let me distinguish Smad2 and Smad3 in paraffin sections of mouse heart. What would you recommend?
Asked on Oct 19 2012
Thank you for contacting us.
We do have a number of anti-SMAD2 and anti-SMAD3 antibodies which we guarantee in IHC-P on mouse. We to don't have the opportunity to test specific products against one another in each application but based on Abreviews, publications and other data available on our datasheets I can recommend the following products.
For identifying both SMAD2 and SMAD3 in IHC-P https://www.abcam.com/Smad2-Smad3-antibody-ab65847.html https://www.abcam.com/Smad2-Smad3-antibody-ab65847.html is guaranteed by our Abpromise in mouse.
While we have not tested our SMAD2 products for SMAD3 reactivity or the SMAD3 against SMAD2, I can recommend the following products for identifying the specific proteins based on sequence homologies.
For identifying only SMAD2 in IHC-P we recommend the rabbit monoclonal https://www.abcam.com/Smad2-antibody-EP567Y-ab33875.html https://www.abcam.com/Smad2-antibody-EP567Y-ab33875.html. The immunogen used for this product has only a 44.4% identity to SMAD3 Mouse.
For identifying only SMAD2 in IHC-P we recommend the rabbit monoclonal https://www.abcam.com/Smad3-antibody-EP568Y-ab40854.html https://www.abcam.com/Smad3-antibody-EP568Y-ab40854.html. The immunogen used for this product has only a 47.4% identity to SMAD2 Mouse.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Answered on Oct 19 2012